15N labeling of amino acids in Spirodela Polyrhiza

Abstract

These data comprise15N stable isotopic labeling study of amino acids in Spirodela polyrhiza (common duckweed) grown under three different light and carbon input conditions which represent unique potential metabolic modes. Plants were grown with a light cycle, either with supplemental sucrose (mixotrophic) or without supplemental sucrose (photoautotrophic) and in the dark with supplemental sucrose (heterotrophic). In this study, stable isotopic labeling with 15N of S. polyrhiza allowed for estimation of amino acid pool sizes, turnover, and kinetics.