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Recent Submissions
Survey Results from A Co-Produced Study Engaging Water Utility Frontline Workers
(2024-07-15) Gonzales, Katerina R.; Roop, Heidi A.; Rozance, Mary Ann; Grodnik-Nagle, Ann; Purnell, Danielle; Rack, Marieke; Branam, Easton
This study engaged frontline crews at Seattle Public Utilities (SPU) to understand and inform how future frontline workforce engagement can be integrated in adaptation planning and investments including frontline crew engagement as a necessary part of a robust and equitable climate adaptation planning. Water utility crews are frontline workers because they may be directly exposed to climate impacts where they work. These frontline workers experience climate change impacts, including heavier rainfall, firsthand. This exposure has implications for climate equity. In Seattle, Washington, the City has made equity plans, community engagement plans, and climate action and adaptation plans, yet to-date none of them explicitly include frontline workers' knowledge, experiences, or exposure as critical to understanding and managing climate change. Frontline workers are not just impacted by climate-amplified hazards, but they are also implementers of climate adaptation strategies. These workers hold experience-based knowledge about the effective management of water utility systems and knowledge of current climate adaptation strategies. This study, through direct frontline worker engagement, explores how water utilities can intentionally include crews' on-the-ground perspectives in adaptation efforts and invest in increasing workers' ability to adapt as part of reciprocity for incorporating their experiential knowledge into climate adaptation planning and action. The data included herein are from a survey distributed to frontline water utility staff at the beginning of the study. The survey includes questions related to participants' perceptions of climate change impacts, anticipation of future climate impacts, changes to staff responses, and perceptions of needs that would enhance their adaptive capacity. Data are both qualitative (short answer), and quantitative (multiple choice).
Nuclear Magnetic Resonance and Circular Dichroism Spectroscopy to study the folding state of artificial, primordial-like proteins
(2024-07-25) Blascyk, Eli
Proteins are the primary molecular machinery for all known life. All proteins are made from the standard 20 amino acids in accordance to the universal genetic code of life; however, prior to the last universal common ancestor, this code was not set in stone and had to evolve to include each of the amino acids. The Seelig lab has previously generated libraries of random proteins using reduced sets of amino acids in order to simulate possible early protein functionality. The reduced sets of amino acids were based on the consensus chronology of incorporation into the genetic code and consisted of 5, 9, 16, and 20 of the amino acids used in modern proteins. This research aimed to characterize the structures of ATP binding proteins from each mRNA display library, primarily focusing on the 5AA and 9AA alphabet libraries. HSQC NMR and CD spectroscopy were used to characterize the structural and folding state of these proteins. Measurements from these spectroscopy experiments reveal secondary structure characteristics of up to 45% alpha helicity for individual variants. Further characterization efforts for these proteins are ongoing, including additional NMR and CD experiments as well as attempts to crystallize the proteins for X-ray crystallography.
Department of Theatre Handbook 2009-2011
(2009) University of Minnesota Duluth. Department of Theatre
UMD Theatre Season Brochure (2010-2011 Season)
(2010) University of Minnesota Duluth. UMD Theatre