Data for Extracellular Phosphate Modulation and Polyphosphate Accumulation by Corynebacterium matruchotii and Streptococcus mutans
2024-11-21
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11-16-2019
06-24-2020
06-24-2020
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06-24-2020
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Data for Extracellular Phosphate Modulation and Polyphosphate Accumulation by Corynebacterium matruchotii and Streptococcus mutans
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2024-11-21
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Jones, Robert S
rsjones@umn.edu
rsjones@umn.edu
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Abstract
An alternative and understudied microbial mechanism that may influence demineralization is the microbially mediated ion exchange of Ca2+ and orthophosphate (Pi), which alters the saturation state of the mineral species within the surface enamel. There is a need to examine the ability of members of the oral microbiome to modulate Ca2+ and Pi, which control mineral solubility, in order to effectively evaluate mineralization therapies to improve oral health. (2) Methods: Pi uptake was measured using an ascorbic acid assay during a BHI liquid culture growth of Corynebacterium matruchotii and Streptococcus mutans for up to 20 h. The initial and endpoint medium Ca2+ levels were measured using ICP-OES. Bacterial cells were examined at different growth stages using DAPI/polyP binding emission at 525 nm to detect the presence of internalized macromolecules of polyphosphates (polyP) that could drive Pi uptake. (3) Results: C. matruchotii (p = 0.0061) substantially accumulated Pi (3.84 mmol/L), with a concomitant formation of polyP. In contrast, S. mutans did not take up Pi or accumulate polyP. No significant Ca2+ drawdown in the media was observed in either strain. (4) Conclusions: This study suggests that when examining the future efficacy of prevention technologies to improve, in vitro assays may consider including specific oral bacteria capable of substantial Pi uptake.
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Data supporting figures in https://doi.org/10.3390/dj12110366
1. File List
A. Filename: DRUM_Ghose_JonesRS_Fig2A.csv
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B. Filename: DRUM_Ghose_JonesRS_Fig2B.csv
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C. Filename:DRUM_Ghose_JonesRS_Fig2C.csv
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D. Filename:Figure DRUM_Ghose_JonesRS_Fig2D.csv
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E. Filename:DRUM_Ghose_JonesRS_Fig3A.csv
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F. Filename:DRUM_Ghose_JonesRS_Fig3B.csv
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https://doi.org/10.3390/dj12110366
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NIH/NIDCR R01DE027669
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Jones, Debarati; Jones, Robert S. (2024). Data for Extracellular Phosphate Modulation and Polyphosphate Accumulation by Corynebacterium matruchotii and Streptococcus mutans. Retrieved from the Data Repository for the University of Minnesota (DRUM), https://hdl.handle.net/11299/267900.
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