This readme.txt file was generated on 20241124 by Robert S. Jones and edited by data curator 20250319 Recommended citation for the data: Ghose, D., & Jones, R. S. (2025). Data for Extracellular Phosphate Modulation and Polyphosphate Accumulation by Corynebacterium matruchotii and Streptococcus mutans [Data set]. Data Repository for the University of Minnesota (DRUM). https://doi.org/10.13020/KQ9S-WZ49 ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset Data for Extracellular Phosphate Modulation and Polyphosphate Accumulation by Corynebacterium matruchotii and Streptococcus mutans 2. Author Information Principal Investigator Contact Information Name: Robert S Jones Institution: University of Minnesota Address: 515 Delaware Street S.E.,Minneapolis, MN 55455 Email: rsjones@umn.edu ORCID: 0000-0002-9213-3657 Associate or Co-investigator Contact Information Name: Debarati Ghose Institution: Texas A&M University: College Station, Texas, US Address: College Station, TX 77843-3258, 123 HELD Email: dghose@tamu.edu ORCID: 0000-0002-4924-743X 3. Date published or finalized for release: 2024 November 21 4. Date of data collection (single date, range, approximate date) 2019-11-16 thru 2020-06-24 5. Geographic location of data collection (where was data collected?): Minneapolis, MN, USA 6. Information about funding sources that supported the collection of the data: NIH/NIDCR R01DE027669 7. Overview of the data (abstract): An alternative and understudied microbial mechanism that may influence demineralization is the microbially mediated ion exchange of Ca2+ and orthophosphate (Pi), which alters the saturation state of the mineral species within the surface enamel. There is a need to examine the ability of members of the oral microbiome to modulate Ca2+ and Pi, which control mineral solubility, in order to effectively evaluate mineralization therapies to improve oral health. Pi uptake was measured using an ascorbic acid assay during a BHI liquid culture growth of Corynebacterium matruchotii and Streptococcus mutans for up to 20 h. The initial and endpoint medium Ca2+ levels were measured using ICP-OES. Bacterial cells were examined at different growth stages using DAPI/polyP binding emission at 525 nm to detect the presence of internalized macromolecules of polyphosphates (polyP) that could drive Pi uptake. C. matruchotii (p = 0.0061) substantially accumulated Pi (3.84 mmol/L), with a concomitant formation of polyP. In contrast, S. mutans did not take up Pi or accumulate polyP. No significant Ca2+ drawdown in the media was observed in either strain. This study suggests that when examining the future efficacy of prevention technologies to improve, in vitro assays may consider including specific oral bacteria capable of substantial Pi uptake. Keywords: oral; bacteria; calcium; phosphate; polyphosphate -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: CC0 1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/deed.en 2. Links to publications that cite or use the data: Ghose, D., & Jones, R. S. (2024). Extracellular Phosphate Modulation and Polyphosphate Accumulation by Corynebacterium matruchotii and Streptococcus mutans. Dentistry Journal, 12(11), 366. https://doi.org/10.3390/dj12110366 3. Was data derived from another source? no 4. Terms of Use: Data Repository for the U of Minnesota (DRUM) By using these files, users agree to the Terms of Use. https://conservancy.umn.edu/pages/policies/#drum-terms-of-use --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: DRUM_Ghose_JonesRS_Fig2A.csv Short description: Data for corresponding figure described in file name B. Filename: DRUM_Ghose_JonesRS_Fig2B.csv Short description: Data for corresponding figure described in file name C. Filename:DRUM_Ghose_JonesRS_Fig2C.csv Short description: Data for corresponding figure described in file name D. Filename:Figure DRUM_Ghose_JonesRS_Fig2D.csv Short description: Data for corresponding figure described in file name E. Filename:DRUM_Ghose_JonesRS_Fig3A.csv Short description: Data for corresponding figure described in file name F. Filename:DRUM_Ghose_JonesRS_Fig3B.csv Short description: Data for corresponding figure described in file name 2. Relationship between files: Raw data of all figures and tables in publication and supplmental -------------------------- METHODOLOGICAL INFORMATION -------------------------- All methodological information can be found in the published manuscript which is open access. Anyone can open, view, and download the article. 1. Description of methods used for collection/generation of data: see publication link: https://doi.org/10.3390/dj12110366 2. Methods for processing the data: see publication link: https://doi.org/10.3390/dj12110366 3. Instrument- or software-specific information needed to interpret the data: CSV 4. Standards and calibration information, if appropriate: see publication link: https://doi.org/10.3390/dj12110366 5. Environmental/experimental conditions: see publication link: https://doi.org/10.3390/dj12110366 6. Describe any quality-assurance procedures performed on the data: see publication link: https://doi.org/10.3390/dj12110366 7. People involved with sample collection, processing, analysis and/or submission: sample collection, processing: D. Ghose, and R. Jones