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Supporting data for The fluorescence-activating and absorption-shifting tag (FAST) enables live-cell fluorescence imaging of Methanococcus maripaludis

Hernandez, Eric
Costa, Kyle
2022-05-06
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Persistent link to this item

https://doi.org/10.13020/qwp0-1824
 https://hdl.handle.net/11299/227287
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Collection period

2021-10-21
2021-11-30

Date completed

2021-11-30

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Title

Supporting data for The fluorescence-activating and absorption-shifting tag (FAST) enables live-cell fluorescence imaging of Methanococcus maripaludis

Published Date

2022-05-06

Authors

Group

University of Minnesota Costa Lab

Author Contact

Costa, Kyle
kcosta@umn.edu

Type

Dataset
Experimental Data

Abstract

Microscopy images of Methanococcus maripaludis cells expressing FAST1 tagged to different genes involved in methanogenesis. Images were used for quantification of protein expression in live cells.

Description

.tiff files of M. maripaludis cells expressing FAST1 tagged to different genes involved in methanogenesis. Images include phase contrast and fluorescent channel images acquired with an echo revolve microscope. The image files are 16-bit (to preserve the light intensity information). To display the images properly they should be opened using a program such as ImageJ am open source supported image viewer that can be downloaded from https://imagej.nih.gov/ij/download.html

Referenced by

https://doi.org/10.1101/2022.04.01.486799

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License

CC0 1.0 Universal
 http://creativecommons.org/publicdomain/zero/1.0/

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Data Repository for U of M (DRUM)

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Suggested citation

Hernandez, Eric; Costa, Kyle. (2022). Supporting data for The fluorescence-activating and absorption-shifting tag (FAST) enables live-cell fluorescence imaging of Methanococcus maripaludis. Retrieved from the Data Repository for the University of Minnesota (DRUM), https://doi.org/10.13020/qwp0-1824.

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Readme.txt
Dataset documentation
(4.75 KB)

Fluorescent Analysis Raw Images.zip
Raw .tiff Micrographs
(804.83 MB)

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