This readme.txt file was generated on <20220509> by <Eric Hernandez>


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GENERAL INFORMATION
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1.Title: Supporting data for The fluorescence-activating and absorption-shifting tag (FAST) enables live-cell fluorescence imaging of Methanococcus maripaludis


2. Author Information
	
  Principal Investigator Contact Information
        Name: Kyle Costa
           Institution: University of Minnesota Twin Cities 
           Email: kcosta@umn.edu
	   

  Associate or Co-investigator Contact Information
        Name: Eric Hernandez
           Institution: University of Minnesota Twin Cities
           Email: hern0421@umn.edu
	    

3. Date published or finalized for release: 
	20220506

4. Date of data collection 
	20221021 - 20221130

5. Geographic location of data collection (where was data collected?): 
	N/A 

6. Information about funding sources that supported the collection of the data: 
	N/A

7. Overview of the data (abstract): 
	Microscopy images of Methanococcus maripaludis cells expressing FAST1 tagged to different genes involved in methanogenesis. Images were used for quantification of protein expression in live cells.

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SHARING/ACCESS INFORMATION
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1. Licenses/restrictions placed on the data: 
	CC0 1.0 Universal

2. Links to publications that cite or use the data: 
	https://doi.org/10.1101/2022.04.01.486799

3. Was data derived from another source?
           If yes, list source(s): N/A

4. Terms of Use: Data Repository for the U of Minnesota (DRUM) By using these files, users agree to the Terms of Use. https://conservancy.umn.edu/pages/drum/policies/#terms-of-use

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DATA & FILE OVERVIEW
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Images file names contain metadata such as growth OD, unofficial genotype name, exposure, and whether or not the samples were pictures with
fluorogen.

See below for example:
"peh4-formate-nowash-od247-hg-995ms-wf_0000_fitc"

The file name above is broken down as follows:
peh4 is the unofficial name of the N-terminally tagged FruA strain. Pictured cells were grown in formate,unwashed, pictured at an OD600
of 247, high gain image setting, 995ms exposure, and WF refers to WITH Fluorogen (NF denotes WITHOUT Fluorogen), the 4 digit number string
represents an arbitrarily numbered field of view from the sample, and FITC denotes fluorescent channel (TRANS indicates transmitted light/phase
contrast)

A. FruA Micrographs Folder contains images of M. maripaludis expressing an N-terminal FAST1 tag on FruA. 
	Formate Grown and Hydrogen Grown folders contain images grouped by biological replicates of log phase cells grown in media
	in which either formate or hydrogen was the sole electron donor, respectively. 
B. Fdh SplitFAST Micrographs folder contain images of M. maripaludis expressing c-terminal fusions of NFAST and CFAST.
	FdhA+FdhB folder contains cells expressing FdhA tagged with NFAST and FdhB tagged with CFAST. FdhA+Mtd folder contains images of cells
	expressing FdhA tagged with NFAST and Mtd taged with CFAST. Mtd+FdhB folder contains cells expresing Mtd tagged with NFAST and
	FdhB tagged with CFAST. These images are split into folders named and represented by different biological replicates. 
	The FdhA+FdhB folder is further subdivided into conditions in which this cell type was imaged grown under hydrogen containing
	media or formate containg media.

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METHODOLOGICAL INFORMATION
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1. Description of methods used for collection/generation of data: 
	Cells were grown to exponential phase in in liquid media containing either hydrogen or formate as specified above and treated with
	HMBR to a concentration of 10 micromolar in an anaerobic prep. Images were taken with an echo revolve microscope in anoxy.
	Images are raw tiff files directly from the native microscope software. They are unprocessed.

2. Methods for processing the data: N/A


3. Instrument- or software-specific information needed to interpret the data:
	Echo Revolve Microscope
The image files are 16-bit (to preserve the light intensity information). To display the images properly they should be opened using a program such as ImageJ am open source supported image viewer that can be downloaded from https://imagej.nih.gov/ij/download.html 


4. Standards and calibration information, if appropriate: N/A


5. Environmental/experimental conditions:
	Images were taken in an anaerobic chamber with 3% hydrogen, nitrogen balance. 

6. Describe any quality-assurance procedures performed on the data:
N/A

7. People involved with sample collection, processing, analysis and/or submission:
	Eric Hernandez
	Kyle Costa




4. Variable List
                  
	N/A