Fluoride and Gallein Inhibit Polyphosphate Accumulation by Oral Pathogen Rothia dentocariosa - Data Sharing Archive
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2021-5-01
2022-7-31
2022-7-31
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2022-1-16
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Fluoride and Gallein Inhibit Polyphosphate Accumulation by Oral Pathogen Rothia dentocariosa - Data Sharing Archive
Published Date
2023-01-25
Author Contact
Jones, Robert, S
rsjones@umn.edu
rsjones@umn.edu
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Experimental Data
Abstract
This raw data set supports publication found in Letters in Applied Microbiology:
The uptake and storage of extracellular orthophosphate (Pi) by polyphosphate (polyP) accumulating bacteria may contribute to mineral dissolution in the oral cavity. To test the effect of potential inhibitors of polyP kinases on Rothia dentocariosa, gallein (0, 25, 50, 100 µM) and fluoride (0, 50, 100 ppm) were added to R. dentocariosa cultures grown in brain heart infusion broth. At late log growth phase (8h), extracellular Pi was measured using an ascorbic acid assay, and polyP was isolated from bacterial cells treated with RNA/DNAases using a neutral phenol/chloroform extraction. Extracts were hydrolyzed and quantified as above. Gallein and fluoride had minor effects on bacterial growth with NaF having a direct effect on media pH. Gallein (≥25 µM) and fluoride (≥50 ppm) attenuated the bacterial drawdown of extracellular Pi 56.7% (p <0.05) and 37.3% (p <0.01). There was a corresponding polyP synthesis decrease of 73.2% (p<0.0001) from gallein and 83.1% (p<0.0001) from fluoride. Attenuated total reflectance Fourier transform infrared spectroscopy validated the presence of polyP and its reduced concentration in R. dentocariosa bacterial cells following gallein and fluoride treatment. R. dentocariosa can directly change extracellular Pi and accumulate intracellular polyP but the mechanism is attenuated by gallein and NaF.
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Protocol Description, Raw Data Results, and Figure Data from collaboration between Earth and Environmental Science and the School of Dentistry.
Referenced by
Dhiraj Kumar, Subhrangshu Mandal, Jake V Bailey, Beverly E Flood, Robert S Jones, Fluoride and Gallein Inhibit Polyphosphate Accumulation by Oral Pathogen Rothia dentocariosa, Letters in Applied Microbiology, 2023;, ovad017, https://doi.org/10.1093/lambio/ovad017
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NIH/NIDCR R01DE027669
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Kumar, Dhiraj; Mandal, Subhrangshu; Bailey, Jake V.; Flood, Beverly E.; Jones, Robert S.. (2023). Fluoride and Gallein Inhibit Polyphosphate Accumulation by Oral Pathogen Rothia dentocariosa - Data Sharing Archive. Retrieved from the Data Repository for the University of Minnesota (DRUM), https://doi.org/10.13020/d7wn-7n44.
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Figure 2A_Gallein treatment - Growth curve.xlsx
Figure Data
(9.83 KB)
Figure 2A_Gallein treatment - pH.xlsx
Figure Data
(9.38 KB)
Figure 2B_Fluoride treatment - Growth curve.xlsx
Figure Data
(9.71 KB)
Figure 3A_PO4 Drawn from media - Gallein treatment.xlsx
(9.19 KB)
Figure 2B_Fluoride treatment - pH.xlsx
Figure Data
(9.27 KB)
Figure 3B_PO4 Drawn from media - Fluoride treatment.xlsx
Figure Data
(9.12 KB)
Figure 3C_PolyP quantification with Gallein treatment.xlsx
Figure Data
(9.18 KB)
Figure 3D_PolyP quantification with Fluoride treatment.xlsx
(9.17 KB)
Figure 4A_FTIR data for std PolyP P45 and P100.xlsx
Figure Data
(382.21 KB)
Figure 4B_FTIR data for PolyP isolated from Rd post Gallein treatment.xlsx
Figure Data
(711.81 KB)
Figure 4C_FTIR data for PolyP isolated from Rd post Fluoride treatment.xlsx
Figure Data
(624.86 KB)
Figure 5A_Fluoride-Gallein combo Growth.xlsx
Figure Data
(9.42 KB)
Figure 5A_Fluoride-Gallein combo pH.xlsx
Figure Data
(9.33 KB)
Figure 5B_Fluoride-Gallein combo PO4 Drawn from media.xlsx
Figure Data
(9.11 KB)
Figure 5C_Fluoride-Gallein combo PolyP quantification.xlsx
Figure Data
(9.13 KB)
Figure S1_Rd 24h growth.xlsx
Figure Data-Supplemental
(11.17 KB)
Figure S1_Rd 24h pH.xlsx
Figure Data-Supplemental
(10.81 KB)
Figure S2_PO4 std curve.xlsx
Figure Data-Supplemental
(10.44 KB)
Figure S4_Std PolyP P45 and P100 quantification.xlsx
Figure Data-Supplemental
(9.16 KB)
Duplicate files in csv format for preservation.zip
CSV versions of the files in this data record
(527.3 KB)
Readme_published_V4 (2).txt
Readme File
(4.84 KB)
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