This readme.txt file was generated on 20230131 by Jones, Robert S

Recommended citation for the data: Kumar, Dhiraj; Mandal, Subhrangshu; Bailey, Jake V.; Flood, Beverly E.; Jones, Robert S.. (2023). Fluoride and Gallein Inhibit Polyphosphate Accumulation by Oral Pathogen Rothia dentocariosa - Data Sharing Archive. Retrieved from the University of Minnesota Digital Conservancy, https://hdl.handle.net/11299/250573.


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GENERAL INFORMATION
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1. Title of Dataset : Fluoride and Gallein Inhibit Polyphosphate Accumulation by Oral Pathogen Rothia dentocariosa - Data Sharing Archive


2. Author Information: Authors
Kumar, Dhiraj
Mandal, Subhrangshu
Bailey, Jake V.
Flood, Beverly E.
Jones, Robert S.


  Principal Investigator Contact Information
        Name: Prof. Robert Jones	
           Institution: Department of Surgical and Developmental Sciences, School of Dentistry, University of Minnesota
           Address: 515 Delaware St SE, Minneapolis 55455.
           Email: rsjones@umn.edu	
	   ORCID: orcid.org/0000-0002-9213-3657

  Associate or Co-investigator Contact Information
        Name: Jake V. Bailey
           Institution: Department of Earth & Environmental Sciences
           Address: 
           Email: baileyj@umn.edu
	   ORCID: 

3. Date published or finalized for release: 01/28/2023

4. Date of data collection (single date, range, approximate date) <suggested format YYYYMMDD>: 20210501-2022731

5. Geographic location of data collection (where was data collected?):  17-237 Moos Tower, School of Dentistry, University of Minnesota

6. Information about funding sources that supported the collection of the data: This works is supported by NIH grant NIH/NIDCR R01DE027669.

7. Overview of the data (abstract): 

The uptake and storage of extracellular orthophosphate (Pi) by polyphosphate (polyP)
accumulating bacteria may contribute to mineral dissolution in the oral cavity. To test the effect
of potential inhibitors of polyP kinases on Rothia dentocariosa, gallein (0, 25, 50, 100 μM) and
fluoride (0, 50, 100 ppm) were added to R. dentocariosa cultures grown in brain heart infusion
broth. At late log growth phase (8h), extracellular Pi was measured using an ascorbic acid assay,
and polyP was isolated from bacterial cells treated with RNA/DNAases using a neutral
phenol/chloroform extraction. Extracts were hydrolyzed and quantified as above. Gallein and
fluoride had minor effects on bacterial growth with NaF having a direct effect on media pH.
Gallein (≥25 μM) and fluoride (≥50 ppm) attenuated the bacterial drawdown of extracellular Pi
56.7% (p <0.05) and 37.3% (p <0.01). There was a corresponding polyP synthesis decrease of
73.2% (p<0.0001) from gallein and 83.1% (p<0.0001) from fluoride. Attenuated total reflectance
Fourier transform infrared spectroscopy validated the presence of polyP and its reduced
concentration in R. dentocariosa bacterial cells following gallein and fluoride treatment. R.
dentocariosa can directly change extracellular Pi and accumulate intracellular polyP but the
mechanism is attenuated by gallein and NaF.


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SHARING/ACCESS INFORMATION
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1. Licenses/restrictions placed on the data: 
Attribution-NonCommercial-NoDerivs 3.0 United States
https://creativecommons.org/licenses/by-nc-nd/3.0/us/

2. Links to publications that cite or use the data: https://academic.oup.com/lambio/advance-article/doi/10.1093/lambio/ovad017/7008850

3. Was data derived from another source? No
           If yes, list source(s):

4. Terms of Use: Data Repository for the U of Minnesota (DRUM) By using these files, users agree to the Terms of Use. https://conservancy.umn.edu/pages/drum/policies/#terms-of-use


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METHODOLOGICAL INFORMATION
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1. Description of methods used for collection/generation of data: Please refer to publication "https://doi.org/10.1093/lambio/ovad017".

2. Methods for processing the data: <describe how the submitted data were generated from the raw or collected data> Please refer to publication "https://doi.org/10.1093/lambio/ovad017".


3. Instrument- or software-specific information needed to interpret the data: Please refer to publication "https://doi.org/10.1093/lambio/ovad017".


4. Standards and calibration information, if appropriate: Please refer to publication "https://doi.org/10.1093/lambio/ovad017".


5. Environmental/experimental conditions: Please refer to publication "https://doi.org/10.1093/lambio/ovad017".


6. Describe any quality-assurance procedures performed on the data: Please refer to publication "https://doi.org/10.1093/lambio/ovad017".


7. People involved with sample collection, processing, analysis and/or submission: Please refer to publication "https://doi.org/10.1093/lambio/ovad017".