Raw data for Xyloglucan deficiency leads to a reduction in turgor pressure and changes in cell wall properties affecting early seedling establishment

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https://doi.org/10.13020/e0jh-4k93
https://hdl.handle.net/11299/262082

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Keywords

Cell wall

xyloglucan

turgor pressure

seedling emergence

root penetration

wall mechanics

Collection period

2019-12-01
2024-03-07

Date completed

2024-03-28

Date updated

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Geographic coverage

Source information

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Volume Title

Title

Raw data for Xyloglucan deficiency leads to a reduction in turgor pressure and changes in cell wall properties affecting early seedling establishment

Published Date

2024-04-08

Authors

Bou Daher, Firas

Serra, Leo

Carter, Ross

Jönsson, Henrik

Robinson, Sarah

Meyerowitz, Elliot M

Gray, William M

Group

William Gray Lab

Author Contact

Gray, William M
grayx051@umn.edu

Type

Dataset
Experimental Data

Abstract

Xyloglucan is believed to play a significant role in cell wall mechanics of dicot plants. Surprisingly, Arabidopsis plants defective in xyloglucan biosynthesis exhibit nearly normal growth and development. We investigated a mutant line, cslc-Δ5, lacking activity in all five Arabidopsis CSLC genes responsible for xyloglucan backbone biosynthesis. We observed that this xyloglucandeficient line exhibited reduced cellulose crystallinity and increased pectin levels, suggesting the existence of feedback mechanisms that regulate wall composition to compensate for the absence of xyloglucan. These alterations in cell wall composition in the xyloglucan-absent plants were further linked to a decrease in cell wall elasticity and rupture stress, as observed through atomic force microscopy and extensometer-based techniques. This raised questions about how plants with such modified cell wall properties can maintain normal growth. Our investigation revealed two key factors contributing to this phenomenon. Firstly, measurements of turgor pressure, a primary driver of plant growth, revealed that cslc-Δ5 plants have reduced turgor, preventing the compromised walls from bursting while still allowing growth to occur. Secondly, we discovered the conservation of elastic asymmetry (ratio of axial to transverse wall elasticity) in the mutant, suggesting an additional mechanism contributing to the maintenance of normal growth. This novel feedback mechanism between cell wall composition and mechanical properties, coupled with turgor pressure regulation, plays a central role in the control of plant growth and is critical for seedling establishment in a mechanically challenging environment by affecting shoot emergence and root penetration.

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Referenced by

Firas Bou Daher, Leo Serra, Ross Carter, Henrik Jönsson, Sarah Robinson, Elliot M. Meyerowitz, William M. Gray. (2024). Xyloglucan deficiency leads to a reduction in turgor pressure and changes in cell wall properties, affecting early seedling establishment. Current Biology; In Press
https://doi.org/10.1016/j.cub.2024.04.016

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Attribution-NonCommercial-NoDerivs 3.0 United States
http://creativecommons.org/licenses/by-nc-nd/3.0/us/

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Data Repository for U of M (DRUM)

Funding information

NIH GM145298
NIH GM067203
Gatsby Charitable Trust GAT3731/DAA
Howard Hughes Medical Institute
Royal Society URF URF\R1\180196
Gatsby charitable foundation GAT3731/GLJ
Leverhulme Trust RPG-2022-111

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Suggested citation

Bou Daher, Firas; Serra, Leo; Carter, Ross; Jönsson, Henrik; Robinson, Sarah; Meyerowitz, Elliot M; Gray, William M. (2024). Raw data for Xyloglucan deficiency leads to a reduction in turgor pressure and changes in cell wall properties affecting early seedling establishment. Retrieved from the University Digital Conservancy, https://doi.org/10.13020/e0jh-4k93.

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