Raw data for Xyloglucan deficiency leads to a reduction in turgor pressure and changes in cell wall properties affecting early seedling establishment
2024-04-08
Loading...
Persistent link to this item
Statistics
View StatisticsCollection period
2019-12-01
2024-03-07
2024-03-07
Date completed
2024-03-28
Date updated
Time period coverage
Geographic coverage
Source information
Journal Title
Journal ISSN
Volume Title
Title
Raw data for Xyloglucan deficiency leads to a reduction in turgor pressure and changes in cell wall properties affecting early seedling establishment
Published Date
2024-04-08
Group
Author Contact
Gray, William M
grayx051@umn.edu
grayx051@umn.edu
Type
Dataset
Experimental Data
Experimental Data
Abstract
Xyloglucan is believed to play a significant role in cell wall mechanics of dicot plants.
Surprisingly, Arabidopsis plants defective in xyloglucan biosynthesis exhibit nearly
normal growth and development. We investigated a mutant line, cslc-Δ5, lacking
activity in all five Arabidopsis CSLC genes responsible for xyloglucan backbone
biosynthesis. We observed that this xyloglucandeficient line exhibited reduced
cellulose crystallinity and increased pectin levels, suggesting the existence of feedback
mechanisms that regulate wall composition to compensate for the absence of
xyloglucan. These alterations in cell wall composition in the xyloglucan-absent plants
were further linked to a decrease in cell wall elasticity and rupture stress, as observed
through atomic force microscopy and extensometer-based techniques. This raised
questions about how plants with such modified cell wall properties can maintain normal
growth. Our investigation revealed two key factors contributing to this phenomenon.
Firstly, measurements of turgor pressure, a primary driver of plant growth, revealed
that cslc-Δ5 plants have reduced turgor, preventing the compromised walls from
bursting while still allowing growth to occur. Secondly, we discovered the conservation
of elastic asymmetry (ratio of axial to transverse wall elasticity) in the mutant,
suggesting an additional mechanism contributing to the maintenance of normal growth.
This novel feedback mechanism between cell wall composition and mechanical
properties, coupled with turgor pressure regulation, plays a central role in the control of
plant growth and is critical for seedling establishment in a mechanically challenging
environment by affecting shoot emergence and root penetration.
Description
Referenced by
Firas Bou Daher, Leo Serra, Ross Carter, Henrik Jönsson, Sarah Robinson, Elliot M. Meyerowitz, William M. Gray. (2024). Xyloglucan deficiency leads to a reduction in turgor pressure and changes in cell wall properties, affecting early seedling establishment. Current Biology; In Press
https://doi.org/10.1016/j.cub.2024.04.016
https://doi.org/10.1016/j.cub.2024.04.016
Related to
Replaces
item.page.isreplacedby
Publisher
Collections
Funding information
NIH GM145298
NIH GM067203
Gatsby Charitable Trust GAT3731/DAA
Howard Hughes Medical Institute
Royal Society URF URF\R1\180196
Gatsby charitable foundation GAT3731/GLJ
Leverhulme Trust RPG-2022-111
NIH GM067203
Gatsby Charitable Trust GAT3731/DAA
Howard Hughes Medical Institute
Royal Society URF URF\R1\180196
Gatsby charitable foundation GAT3731/GLJ
Leverhulme Trust RPG-2022-111
item.page.sponsorshipfunderid
item.page.sponsorshipfundingagency
item.page.sponsorshipgrant
Previously Published Citation
Other identifiers
Suggested citation
Bou Daher, Firas; Serra, Leo; Carter, Ross; Jönsson, Henrik; Robinson, Sarah; Meyerowitz, Elliot M; Gray, William M. (2024). Raw data for Xyloglucan deficiency leads to a reduction in turgor pressure and changes in cell wall properties affecting early seedling establishment. Retrieved from the Data Repository for the University of Minnesota (DRUM), https://doi.org/10.13020/e0jh-4k93.
View/Download File
File View/Open
Description
Size
Figure1_S1_M1.zip
Raw data for Figures 1 and S1
(352.31 MB)
Figure2_S2.zip
Raw data for Figures 2 and S2
(1.26 GB)
Figure3_S3.zip
Raw data for Figures 3 and S3
(3.87 GB)
Figure4_S4_M2-3-4.zip
Raw data for Figures 4 and S4 and movies 2, 3, 4
(63.58 MB)
Figure5_S5.zip
Raw data for Figures 5 and S5
(139.02 MB)
Bou Daher et al_2024_Pre-print.pdf
Pre-print of Bou Daher et al. Current Biology paper
(2.94 MB)
BouDaher_2024_Readme.txt
Description of the data
(14.26 KB)
Archival data.zip
Archival data (CSV format)
(735.68 KB)
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.