This readme.txt file was generated on <20240330> by Recommended citation for the data: Bou Daher, Firas; Serra, Leo; Carter, Ross; Jönsson, Henrik; Robinson, Sarah; Meyerowitz, Elliot M; Gray, William M. (2024). Raw data for Xyloglucan deficiency leads to a reduction in turgor pressure and changes in cell wall properties affecting early seedling establishment. Retrieved from the Data Repository for the University of Minnesota, https://doi.org/10.13020/e0jh-4k93. ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset Raw data for the paper "Xyloglucan deficiency leads to a reduction in turgor pressure and changes in cell wall properties affecting early seedling establishment" 2. Author Information Principal Investigator Contact Information Name:William M. Gray Institution: University of Minnesota Address: Email: grayx051@umn.edu ORCID: 0000-0002-1320-290X Associate or Co-investigator Contact Information Name: Firas Bou Daher Institution: University of Minnesota Address: Email: boudaher@umn.edu ORCID: 0000-0002-7493-8662 Associate or Co-investigator Contact Information Name: Leo Serra Institution: University of Cambridge Address: Email: l.serra@imperial.ac.uk ORCID: Associate or Co-investigator Contact Information Name: Sarah Robinson Institution: University of Cambridge Address: Email: sarah.robinson@slcu.cam.ac.uk ORCID: Associate or Co-investigator Contact Information Name: Ross Carter Institution: University of Cambridge Address: Email: rosscarter33@gmail.com ORCID: Associate or Co-investigator Contact Information Name: Henrik Jönsson Institution: University of Cambridge Address: Email: henrik.jonsson@slcu.cam.ac.uk ORCID: Associate or Co-investigator Contact Information Name: Elliot M. Meyerowitz Institution: California Institute of Technology Address: Email: meyerow@caltech.edu ORCID: 3. Date published or finalized for release: 20240426 4. Date of data collection (single date, range, approximate date) 20191201-20240307 5. Geographic location of data collection (where was data collected?): University of Minnesota, USA; University of Cambridge, UK. 6. Information about funding sources that supported the collection of the data: This work was supported by grants from the National Institutes of Health (grants GM145298 and GM067203 to WMG), the Gatsby Charitable Trust (GAT3731/DAA to EMM). The Meyerowitz Laboratory has funding from the Howard Hughes Medical Institute. SR funded by Royal Society URF URF\R1\180196 and Gatsby Charitable Foundation GAT3731/GLJ. LS funded by Leverhulme Trust RPG-2022-111. 7. Overview of the data (abstract): Xyloglucan is believed to play a significant role in cell wall mechanics of dicot plants. Surprisingly, Arabidopsis plants defective in xyloglucan biosynthesis exhibit nearly normal growth and development. We investigated a mutant line, cslc-Δ5, lacking activity in all five Arabidopsis CSLC genes responsible for xyloglucan backbone biosynthesis. We observed that this xyloglucan-deficient line exhibited reduced cellulose crystallinity and increased pectin levels, suggesting the existence of feedback mechanisms that regulate wall composition to compensate for the absence of xyloglucan. These alterations in cell wall composition in the xyloglucan-absent plants were further linked to a decrease in cell wall elasticity and rupture stress, as observed through atomic force microscopy and extensometer-based techniques. This raised questions about how plants with such modified cell wall properties can maintain normal growth. Our investigation revealed two key factors contributing to this phenomenon. Firstly, measurements of turgor pressure, a primary driver of plant growth, revealed that cslc-Δ5 plants have reduced turgor, preventing the compromised walls from bursting while still allowing growth to occur. Secondly, we discovered the conservation of elastic asymmetry (ratio of axial to transverse wall elasticity) in the mutant, suggesting an additional mechanism contributing to the maintenance of normal growth. This novel feedback mechanism between cell wall composition and mechanical properties, coupled with turgor pressure regulation, plays a central role in the control of plant growth and is critical for seedling establishment in a mechanically challenging environment by affecting shoot emergence and root penetration. -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: Attribution-NonCommercial-NoDerivs 3.0 United States (https://creativecommons.org/licenses/by-nc-nd/3.0/us/) 2. Links to publications that cite or use the data: Firas Bou Daher, Leo Serra, Ross Carter, Henrik Jönsson, Sarah Robinson, Elliot M. Meyerowitz, William M. Gray. (2024). Xyloglucan deficiency leads to a reduction in turgor pressure and changes in cell wall properties, affecting early seedling establishment. Current Biology; In Press; DOI:https://doi.org/10.1016/j.cub.2024.04.016 3. Was data derived from another source? No 4. Terms of Use: Data Repository for the U of Minnesota (DRUM) By using these files, users agree to the Terms of Use. https://www.google.com/url?q=https://www.google.com/url?q%3Dhttps://conservancy.umn.edu/pages/drum/policies/%2523terms-of-use%26source%3Dgmail-imap%26ust%3D1714141777000000%26usg%3DAOvVaw1XCBP33rfO-XeHIyqUJ1ri&source=gmail-imap&ust=1715023276000000&usg=AOvVaw0plUsB27FNiq-pAdpl4A2G --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: Figure1_S1_M1 Short description: Raw data for Figure1, Figure S1 and Movie M1 B. Filename: Figure2_S2 Short description: Raw data for Figure2 and Figure S2 C. Filename: Figure3_S3 Short description: Raw data for Figure3 and Figure S3 D. Filename: Figure4_S4_M2-3-4 Short description: Raw data for Figure4, Figure S4 and Movies M2, M3 and M4 E. Filename: Figure5_S5 Short description: Raw data for Figure5 and Figure S5 F. Filename: Bou Daher et al_2024_Pre-print Short description: preprint of Current Biology paper -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: Detailed description of the methods used can be found in the Current Biology paper or the preprint included with this submission. 2. Methods for processing the data: Detailed description of the methods used can be found in the Current Biology paper or the preprint included with this submission. 3. Instrument- or software-specific information needed to interpret the data: All imaging data can be opened using ImageJ and AFM data can be opened using JPKSPM Data Processing software 4. Standards and calibration information, if appropriate: NA 5. Environmental/experimental conditions: Temperature and light conditions are described in the Current Biology paper or the preprint included with this submission. 6. Describe any quality-assurance procedures performed on the data: NA 7. People involved with sample collection, processing, analysis and/or submission: F.B., W.M.G, S.R., E.M.M. and H.J. were responsible for conceptualization. F.B., L.S., S.R., R.C. and W.M.G. performed research. H.J., S.R., E.M.M. and W.M.G. were responsible for supervision and funding acquisition. F.B. wrote the original draft. All authors reviewed and edited the manuscript. ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Figure1_S1_M1 ----------------------------------------- Folder directory: Figure1_S1_M1 |_ Immuno pectin |_ [original confocal files for the immunolabelling experiment of Col and cslc-d5 using LM19 and LM20 antibodies] |_ 230621 CCRC-M1_XYGL_2min_b.tif [dot blot image of the xyloglucan extracts in the order presented. Left is XYGL-I and right is XYGL-II] |_ 230621 XYGL immuno quantification.xls [signal intensity of the XYGL immuno quantification] |_ 230626 Pectin cslc xxt.xls [Uronic acid quantification of the pectin extract from AIR. Each sample has 3 replicates. The galacturonic acid calibration curve is also presented] |_ 240214 Pectin esterification.xls [AIR quantification, galacturonic acid curve, esterification levels of 3 replicates per sample presented as methanol produced ] |_ C0_Col-left_d5-right.tif [A TIFF file for a time-lapse of Col (left) and cslc-d5 (right) growth in the dark using IR imaging] |_ C1_Col-left-d5-right.tif [A TIFF file for a time-lapse of Col (left) and cslc-d5 (right) growth in the dark using IR imaging] |_ Pectin_cslc.xls [uronic acid per mg AIR for each sample] 1. Number of variables:3 2. Variable List A. Name: Description: B. Name: Description: C. Name: Description: ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Figure2_S2 ----------------------------------------- File directory: |_ 240304 CBM dots cellulose-XYGL |_ CBM2a [original images for the confocal imaging of Col and cslc-d5 probed with CBM2a dots] |_ CBM28 [original data for the confocal imaging of Col and cslc-d5 probed with CBM28 dots] |_ mock2 [original data for the confocal imaging of the mock treatment] |_ 240304 CBM dots cellulose-XYGL.xls [quantification of the fluorescence signal of the samples probed with CBM2a, CBM28 or the mock treatment] |_ CBM2a_PlotsOfData - Plots all Of the Data (3).xls [statistical analysis of the samples probed with CBM2a and analyzed in "240304 CBM dots cellulose-XYGL.xls"] |_ CBM2a_PlotsOfData_2024-03-05 00_07_20.PNG [plot of the data probed with CBM2a and quantified in "240304 CBM dots cellulose- XYGL.xls"] |_ CBM28_PlotsOfData - Plots all Of the Data (3).xls [statistical analysis of the samples probed with CBM28 and analyzed in "240304 CBM dots cellulose-XYGL.xls"] |_ CBM28_PlotsOfData_2024-03-04 23_51_33.PNG [plot of the data probed with CBM28 and quantified in "240304 CBM dots cellulose- XYGL.xls"] |_ CBM cross sections [original images for the confocal imaging of Col and cslc-d5 probed with CBM2a, CBM28 and the mock] |_ cslc-d5_Col 05nM-isox 72HPG [Folders containing the original brightfield images of Col and cslc-d5 treated with isoxaben or mock] |_ 230710 d5 05-isox 72HPG.xls [quantification of the hypocotyl length and diameter of Col and cslc-d5 treated with isoxaben or mock] |_ 230703 cellulose content cslc-d5.xls [cellulose fraction of AIR from Col and cslc-d5] |_ samples for XYGL and pectin_iodine.xls [XYGL quantification using the iodine method of the samples presented] 1. Number of variables:8 2. Variable List A. Name: Description: B. Name: Description: C. Name: Description: D. Name: Description: E. Name: Description: F. Name: Description: G. Name: Description: H. Name: Description: ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Figure3_S3 ----------------------------------------- File directory: |_ ACME [Contains original folders and csv files from the ACME experiment] |_ AFM [Contains original folders and files from the AFM experiments] |_ Viscosity [Contains original folders and files from the AFM viscosity experiments] |_ 231031 hypo break-strength.xls [contains the data from the break-strength experiment] 1. Number of variables: 5 2. Variable List A. Name: Description: B. Name: Description: