Conformational rearrangements enable iterative backbone 𝑁-methylation in RiPP biosynthesis

Miller, Fredarla S.
Crone, Kathryn K.
Jensen, Matthew R.
Shaw, Sudipta
Harcombe, William R.
Elias, Mikael H.
Freeman, Michael F.
2021-07-30
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https://doi.org/10.13020/y8ry-gm18
 https://hdl.handle.net/11299/222603
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Conformational rearrangements enable iterative backbone 𝑁-methylation in RiPP biosynthesis

Published Date

2021-07-30

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Freeman Lab University of Minnesota

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Freeman, Michael F.
mffreema@umn.edu

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Simulation Data

Abstract

The data deposited here are raw files, R code and mass spec, associated with the results presented in the paper, "Conformational rearrangements enable iterative backbone N-methylation in RiPP biosynthesis." These data have been made publicly available in keeping with the journal's data availability policy.

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R Code: Supplementary Data 1. Code for running kinetics simulations File name: Supplementary Data 1.R Mass Spectrometry .raw files: Supplementary Figure 4a-c. Mass spectrometric analysis of split borosin coexpressions. File name: 20200110_FSM1167_SonA+SonM_24.5min.raw Supplementary Figure 4d-j. Mass spectrometric analysis of split borosin coexpressions. File name: 20200909_KC1063_StrA+StrM_15min_redalk_18pm4.raw Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations. File names: 20200220_FSM1178_kineticmodeling_t=2.raw 20200220_FSM1179_kineticmodeling_t=2.raw 20200220_FSM1180_kineticmodeling_t=8.raw 20200220_FSM1181_kineticmodeling_t=8.raw 20200220_FSM1182_kineticmodeling_t=20.raw 20200220_FSM1183_kineticmodeling_t=20.raw 20200220_FSM1184_kineticmodeling_t=40.raw 20200220_FSM1185_kineticmodeling_t=40.raw 20200220_FSM1186_kineticmodeling_t=60.raw 20200220_FSM1187_kineticmodeling_t=60.raw Supplementary Figure 25a. Mass spectrometric analysis of SonM mutant in vitro reactions. File name: 20190701_kc1007_His-SonA_Y93F.raw Supplementary Figure 25b. Mass spectrometric analysis of SonM mutant in vitro reactions. File name: 20190515_fsm1155_his-SonA-sonMT-R67K.raw Supplementary Figure 25c. Mass spectrometric analysis of SonM mutant in vitro reactions. File name: 20190515_fsm1154_his-sonA_sonMTR67A.raw Supplementary Figure 25d. Mass spectrometric analysis of SonM mutant in vitro reactions. File name: 20190701_kc1008_His-SonA_Y58F.raw Supplementary Figure 25e. Mass spectrometric analysis of SonM mutant in vitro reactions. File name: 20190515_fsm1156_his-sonA-SonMT-Y71F.raw Supplementary Figure 25f. Mass spectrometric analysis of SonM mutant in vitro reactions. File name: 20190701_kc1009_His-SonA_DBLMUT.raw

Referenced by

Miller, F. S., Crone, K. K., Jensen, M. R., Shaw, S., Harcombe, W. R., Elias, M. H., & Freeman, M. F. (2021). Conformational rearrangements enable iterative backbone N-methylation in RiPP biosynthesis. Nature communications, 12(1), 1-14.

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CC0 1.0 Universal
 http://creativecommons.org/publicdomain/zero/1.0/

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Data Repository for U of M (DRUM)

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National Institutes of Health (R35 GM133475 to M.F.F.) and the University of Minnesota along with the BioTechnology Institute (M.F.F., M.H.E., W.A.H.)

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Miller, Fredarla S.; Crone, Kathryn K.; Jensen, Matthew R.; Shaw, Sudipta; Harcombe, William R.; Elias, Mikael H.; Freeman, Michael F.. (2021). Conformational rearrangements enable iterative backbone 𝑁-methylation in RiPP biosynthesis. Retrieved from the Data Repository for the University of Minnesota (DRUM), https://doi.org/10.13020/y8ry-gm18.

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Description
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Supplementary Data 1.R
Supplementary Data 1. Code for running kinetics simulations
(1.5 KB)

20200110_FSM1167_SonA+SonM_24.5min.raw
Supplementary Figure 4a-c. Mass spectrometric analysis of split borosin coexpressions.
(94.77 MB)

20200909_KC1063_StrA+StrM_15min_redalk_18pm4.raw
Supplementary Figure 4d-j. Mass spectrometric analysis of split borosin coexpressions.
(190.01 MB)

20200220_FSM1178_kineticmodeling_t=2.raw
Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations.
(294.77 MB)

20200220_FSM1179_kineticmodeling_t=2.raw
Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations.
(302.7 MB)

20200220_FSM1180_kineticmodeling_t=8.raw
Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations.
(296.53 MB)

20200220_FSM1181_kineticmodeling_t=8.raw
Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations.
(291.86 MB)

20200220_FSM1182_kineticmodeling_t=20.raw
Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations.
(329.07 MB)

20200220_FSM1183_kineticmodeling_t=20.raw
Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations.
(335.34 MB)

20200220_FSM1186_kineticmodeling_t=60.raw
Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations.
(290.11 MB)

20200220_FSM1184_kineticmodeling_t=40.raw
Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations.
(280.61 MB)

20200220_FSM1185_kineticmodeling_t=40.raw
Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations.
(286.1 MB)

20200220_FSM1187_kineticmodeling_t=60.raw
Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations.
(282.3 MB)

20190701_kc1007_His-SonA_Y93F.raw
Supplementary Figure 25a. Mass spectrometric analysis of SonM mutant in vitro reactions.
(49.65 MB)

20190515_fsm1155_his-SonA-sonMT-R67K.raw
Supplementary Figure 25b. Mass spectrometric analysis of SonM mutant in vitro reactions.
(44.75 MB)

20190515_fsm1154_his-sonA_sonMTR67A.raw
Supplementary Figure 25c. Mass spectrometric analysis of SonM mutant in vitro reactions.
(51.08 MB)

20190701_kc1008_His-SonA_Y58F.raw
Supplementary Figure 25d. Mass spectrometric analysis of SonM mutant in vitro reactions.
(52.44 MB)

20190515_fsm1156_his-sonA-SonMT-Y71F.raw
Supplementary Figure 25e. Mass spectrometric analysis of SonM mutant in vitro reactions.
(52.68 MB)

20190701_kc1009_His-SonA_DBLMUT.raw
Supplementary Figure 25f. Mass spectrometric analysis of SonM mutant in vitro reactions.
(50.4 MB)

Readme.txt
Readme
(6.29 KB)

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