This codebook.txt file was generated on 20210804 by wilsonkm ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset Conformational rearrangements enable iterative backbone 𝑁-methylation in RiPP biosynthesis 2. Author Information Associate or Co-investigator Contact Information Name: Fredaria S. Miller Institution: University of Minnesota Associate or Co-investigator Contact Information Name: Kathryn K. Crone Institution: University of Minnesota Associate or Co-investigator Contact Information Name: Matthew R. Jensen Institution: University of Minnesota Associate or Co-investigator Contact Information Name: Sudipta Shaw Institution: University of Minnesota Associate or Co-investigator Contact Information Name: William R. Harcombe Institution: University of Minnesota Principal Investigator Contact Information Name: Mikael H. Elias Institution: University of Minnesota Principal Investigator Contact Information Name: Michael F. Freeman Institution: University of Minnesota Email: mffreeman@umn.edu 3. Date of data collection: 4. Geographic location of data collection (where was data collected?): 5. Information about funding sources that supported the collection of the data: Sponsorship: National Institutes of Health (R35 GM133475 to M.F.F.) and the University of Minnesota along with the BioTechnology Institute (M.F.F., M.H.E., W.A.H.) -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: CC0 1.0 Universal 2. Links to publications that cite or use the data: Miller, F. S., Crone, K. K., Jensen, M. R., Shaw, S., Harcombe, W. R., Elias, M. H., & Freeman, M. F. (2021). Conformational rearrangements enable iterative backbone N-methylation in RiPP biosynthesis. Nature communications, 12(1), 1-14. 3. Recommended citation for the data: Miller, Fredarla S.; Crone, Kathryn K.; Jensen, Matthew R.; Shaw, Sudipta; Harcombe, William R.; Elias, Mikael H.; Freeman, Michael F.. (2021). Conformational rearrangements enable iterative backbone 𝑁-methylation in RiPP biosynthesis. Retrieved from the Data Repository for the University of Minnesota, https://doi.org/10.13020/y8ry-gm18. --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: Supplementary Data 1.R Short description: Supplementary Data 1. Code for running kinetics simulations B. Filename: 20200110_FSM1167_SonA+SonM_24.5min.raw Short description: Supplementary Figure 4a-c. Mass spectrometric analysis of split borosin coexpressions. C. Filename: 20200909_KC1063_StrA+StrM_15min_redalk_18pm4.raw Short description: Supplementary Figure 4d-j. Mass spectrometric analysis of split borosin coexpressions. D. Filename: 20200220_FSM1178_kineticmodeling_t=2.raw Short description: Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations. E. Filename: 20200220_FSM1179_kineticmodeling_t=2.raw Short description: Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations. F. Filename: 20200220_FSM1180_kineticmodeling_t=8.raw Short description: Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations. G. Filename: 20200220_FSM1181_kineticmodeling_t=8.raw Short description: Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations. H. Filename: 20200220_FSM1182_kineticmodeling_t=20.raw Short description: Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations. I. Filename: 20200220_FSM1183_kineticmodeling_t=20.raw Short description: Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations. J. Filename: 20200220_FSM1186_kineticmodeling_t=60.raw Short description: Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations. K. Filename: 20200220_FSM1184_kineticmodeling_t=40.raw Short description: Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations. L. Filename: 20200220_FSM1185_kineticmodeling_t=40.raw Short description: Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations. M. Filename: 20200220_FSM1187_kineticmodeling_t=60.raw Short description: Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and compared to kinetic model simulations. N. Filename: 20190701_kc1007_His-SonA_Y93F.raw Short description: Supplementary Figure 25a. Mass spectrometric analysis of SonM mutant in vitro reactions. O. Filename: 20190515_fsm1155_his-SonA-sonMT-R67K.raw Short description: Supplementary Figure 25b. Mass spectrometric analysis of SonM mutant in vitro reactions. P. Filename: 20190515_fsm1154_his-sonA_sonMTR67A.raw Short description: Supplementary Figure 25c. Mass spectrometric analysis of SonM mutant in vitro reactions. Q. Filename: 20190701_kc1008_His-SonA_Y58F.raw Short description: Supplementary Figure 25d. Mass spectrometric analysis of SonM mutant in vitro reactions. R. Filename: 20190515_fsm1156_his-sonA-SonMT-Y71F.raw Short description: Supplementary Figure 25e. Mass spectrometric analysis of SonM mutant in vitro reactions. S. Filename: 20190701_kc1009_His-SonA_DBLMUT.raw Short description: Supplementary Figure 25f. Mass spectrometric analysis of SonM mutant in vitro reactions. -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: Miller, F. S., Crone, K. K., Jensen, M. R., Shaw, S., Harcombe, W. R., Elias, M. H., & Freeman, M. F. (2021). Conformational rearrangements enable iterative backbone N-methylation in RiPP biosynthesis. Nature communications, 12(1), 1-14. 2. Methods for processing the data: Miller, F. S., Crone, K. K., Jensen, M. R., Shaw, S., Harcombe, W. R., Elias, M. H., & Freeman, M. F. (2021). Conformational rearrangements enable iterative backbone N-methylation in RiPP biosynthesis. Nature communications, 12(1), 1-14.