Browsing by Author "Dong, Yanhong"

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    Analysis of ergosterol in single kernel and ground grain by gas chromatography-mass spectrometry
    (Journal of Agricultural and Food Chemistry, 2006-05-11) Steffenson, Brian; Dong, Yanhong; Mirocha, Chester J
    A method for analyzing ergosterol in a single kernel and ground barley and wheat was developed using gas chromatography−mass spectrometry (GC-MS). Samples were saponified in methanolic KOH. Ergosterol was extracted by “one step” hexane extraction and subsequently silylated by N-trimethylsilylimidazole/trimethylchlorosilane (TMSI/TMCS) reagent at room temperature. The recoveries of ergosterol from ground barley were 96.6, 97.1, 97.1, 88.5, and 90.3% at the levels of 0.2, 1, 5, 10, and 20 μg/g (ppm), respectively. The recoveries from a single kernel were between 93.0 and 95.9%. The precision (coefficient of variance) of the method was in the range 0.8−12.3%. The method detection limit (MDL) and the method quantification limit (MQL) were 18.5 and 55.6 ng/g (ppb), respectively. The ergosterol analysis method developed can be used to handle 80 samples daily by one person, making it suitable for screening cereal cultivars for resistance to fungal infection. The ability for detecting low levels of ergosterol in a single kernel provides a tool to investigate early fungal invasion and to study mechanisms of resistance to fungal diseases.
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    Phenotypic and genotypic data of barley Gen10 population to study a pericentromeric region on chromosome 6H
    (2021-06-01) Yin, Lu; Huang, Yadong; Sallam, Ahmad; Heinen, Shane; Li, Lin; Beaubien, Karen; Dill-Macky, Ruth; Dong, Yanhong; Steffenson, Brian; Smith, Kevin; Muehlbauer, Gary; smith376@umn.edu; Smith, Kevin; University of Minnesota Barley Breeding and Genetics (Smith Lab)
    The phenotypic and genotypic data of the Gen10 population used to study a genetic region on chromosome 6H of barley associated with multiple traits. Gen10 is a population of ~100 Chevron-derived recombinant near-isogenic lines that contained the Fusarisum head blight/kernel discoloration resistance locus from Chevron, a landrace, in the genetic background of Lacey, an elite malting barley variety.