Analysis of ergosterol in single kernel and ground grain by gas chromatography-mass spectrometry

Title

Analysis of ergosterol in single kernel and ground grain by gas chromatography-mass spectrometry

Published Date

2006-05-11

Publisher

Journal of Agricultural and Food Chemistry

Type

Article

Abstract

A method for analyzing ergosterol in a single kernel and ground barley and wheat was developed using gas chromatography−mass spectrometry (GC-MS). Samples were saponified in methanolic KOH. Ergosterol was extracted by “one step” hexane extraction and subsequently silylated by N-trimethylsilylimidazole/trimethylchlorosilane (TMSI/TMCS) reagent at room temperature. The recoveries of ergosterol from ground barley were 96.6, 97.1, 97.1, 88.5, and 90.3% at the levels of 0.2, 1, 5, 10, and 20 μg/g (ppm), respectively. The recoveries from a single kernel were between 93.0 and 95.9%. The precision (coefficient of variance) of the method was in the range 0.8−12.3%. The method detection limit (MDL) and the method quantification limit (MQL) were 18.5 and 55.6 ng/g (ppb), respectively. The ergosterol analysis method developed can be used to handle 80 samples daily by one person, making it suitable for screening cereal cultivars for resistance to fungal infection. The ability for detecting low levels of ergosterol in a single kernel provides a tool to investigate early fungal invasion and to study mechanisms of resistance to fungal diseases.

Description

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Series/Report Number

Funding information

Isbn identifier

Doi identifier

10.1021/jf060149f

Previously Published Citation

Dong, Y., Steffenson, B., & Mirocha, C. (2006). Analysis of ergosterol in single kernel and ground grain by gas chromatography-mass spectrometry. Journal of Agricultural and Food Chemistry, 54(12), 4121-5.

Other identifiers

Suggested citation

Steffenson, Brian; Dong, Yanhong; Mirocha, Chester J. (2006). Analysis of ergosterol in single kernel and ground grain by gas chromatography-mass spectrometry. Retrieved from the University Digital Conservancy, 10.1021/jf060149f.

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