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Data for Membraneless Compartmentalization of Cell-Free Transcription-Translation by Polymer-Assisted Liquid-Liquid Phase Separation

Izri, Ziane
Noireaux, Vincent
2024-11-20
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Persistent link to this item

https://doi.org/10.13020/9gbe-2r60
 https://hdl.handle.net/11299/267840
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Collection period

2019-09-01
2024-08-31

Date completed

2024-08-31

Date updated

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Title

Data for Membraneless Compartmentalization of Cell-Free Transcription-Translation by Polymer-Assisted Liquid-Liquid Phase Separation

Published Date

2024-11-20

Authors

Group

Noireaux Lab

Author Contact

Noireaux, Vincent
noireaux@umn.edu

Type

Dataset
Experimental Data

Abstract

The data shared in this repository consists mainly of the collection of the original fluorescence microscopy pictures of the PSTXTL system presented in the manuscript together with the product of their processing and analysis. Those pictures were acquired through three different channels: Bright Field, FITC and Texas Red. The bright field images inform on the presence of phase-separated droplets, while the FITC and Texas Red channels inform on the content of the phase-separated droplets, depending on the fluorophores used. The results of the fluorometry analysis are also present in the data collection.

Description

Data includes .tif and .xlsx files. These data will allow any manuscript reader full access to the experimental results that lead to the statements and conclusions made in the manuscript. Should the reader try to reproduce the experiments presented in the manuscript, they will have a reference to compare their experimental results.

Referenced by

Izri, Ziane; Noireaux, Vincent. (2024). Membraneless Compartmentalization of Cell-Free Transcription-Translation by Polymer-Assisted Liquid-Liquid Phase Separation. UNDER REVIEW

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License

CC0 1.0 Universal
 http://creativecommons.org/publicdomain/zero/1.0/

Publisher

Collections

Data Repository for U of M (DRUM)

Funding information

National Science Foundation EF-1934496 and CBET-1916030

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Suggested citation

Izri, Ziane; Noireaux, Vincent. (2024). Data for Membraneless Compartmentalization of Cell-Free Transcription-Translation by Polymer-Assisted Liquid-Liquid Phase Separation. Retrieved from the Data Repository for the University of Minnesota (DRUM), https://doi.org/10.13020/9gbe-2r60.

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SI Figures S19-S32 - Table S1 - Annex Figure 2.zip
Data used to make figures S19-S32, Table S1, and Annex Figure 2 of the supplementary information
(2.75 GB)

Readme Izri Noireaux 2024 v2.txt
Description of data
(243.81 KB)

Main Text Figures data.zip
Data for the main text (manuscript) figures.
(480.08 MB)

SI Figures S1-S18.zip
Data used to make figures S1-S18 of the supplementary information
(4.15 GB)

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