Data and analyses files for "Evidence for Complex Interplay between Quorum Sensing and Antibiotic Resistance in Pseudomonas aeruginosa"

Abstract

This data set contains the experimental data, corresponding analysis files and final and intermediary figure files used in the manuscript titled "Interference in microbial signaling highlights the complex relationship between quorum sensing and antibiotic resistance in Pseudomonas aeruginosa" and is intended to be made publicly available for readers and reviewers. It contains the data files from BioTek HTX/Epoch2 Microplate reader for Pseudomonas aeruginosa growth with lactonases/AHLs in Biolog Phenotype MicroArrays(TM) PM11-PM20 and additional replicate experiments done with Pseudomonas aeruginosa growing in Biolog IF-10A media with antibiotics/antimicrobial compounds and lactonases/AHLs. It also contains qPCR data from Thermo Scientific StepOnePlus(TM) Real Time PCR system for assessment of transcription levels of Pseudomonas aeruginosa folA and folP genes. Additionally, all associated and annotated analysis files generated in Microsoft Excel and GraphPad Prism are included. Analysis data from Caenorhabditis elegans killing assays are also included. All the intermediate and final figures that are used in the manuscript are provided as Microsoft PowerPoint files for easy visualization of the workflow and processing.