Raw data for: Biphasic Control of Cell Expansion by Auxin Coordinates Etiolated Seedling Development

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2019-01-01
2021-07-26

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2021-07-26

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Raw data for: Biphasic Control of Cell Expansion by Auxin Coordinates Etiolated Seedling Development

Published Date

2021-07-27

Author Contact

Gray, William M
grayx051@umn.edu

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Experimental Data

Abstract

Seedling emergence is critical for food security. It requires rapid hypocotyl elongation and apical hook formation, both of which are mediated by regulated cell expansion. How these events are coordinated in etiolated seedlings is unclear. Here, we show that biphasic control of cell expansion by the phytohormone auxin underlies this process. Shortly after germination, high auxin levels restrain elongation. This provides a temporal window for apical hook formation, involving a gravity-induced auxin maximum on the eventual concave side of the hook, triggering PP2C.D1-controlled asymmetrical H+-ATPase activity, resulting in differential cell elongation. Subsequently, auxin concentrations decline acropetally and switch from restraining to promoting elongation, driving hypocotyl elongation. Our findings elucidate how differential auxin concentrations throughout the hypocotyl coordinate etiolated development, leading to successful soil emergence.

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Du et al. Science Advances 2021

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National Institutes of Health (GM067203), USDA National Institute of Food and Agriculture (2018-67013-027503), K.C. Wong Education Foundation

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Du, Minmin; Bou Daher, Firas; Liu, Yuanyuan; Steward, Andrew; Tillman, Molly; Zhang, Xiaoyue; Wong, Jeh Haur; Ren, Hong; Cohen, Jerry D; Li, Chuanyou; Gray, William M. (2021). Raw data for: Biphasic Control of Cell Expansion by Auxin Coordinates Etiolated Seedling Development. Retrieved from the Data Repository for the University of Minnesota (DRUM), https://doi.org/10.13020/spr0-2r11.
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Readme.txtDescription of the data7.63 KB
Raw measurements.zipZip file containing raw measurements2.85 MB
Raw images.zipZip file containing raw image data32.02 GB
Raw images2.zipRaw images acquired used in study sorted by figure (Fig S4A and Fig S4B, and movies S5, S6, S7, S8,)659.9 MB

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