Supporting data for Condensable Vapor Sorption by Low Charge State Protein Ions

Lee, Jihyeon
Hogan, Christopher
Clowers, Brian
2022-09-08
Thumbnail Image

Persistent link to this item

https://hdl.handle.net/11299/241499
Statistics
View Statistics

Keywords

Collection period

2021-06-01
2022-01-22

Date completed

2022-01-22

Date updated

Time period coverage

Geographic coverage

Source information

Journal Title

Journal ISSN

Volume Title

Title

Supporting data for Condensable Vapor Sorption by Low Charge State Protein Ions

Published Date

2022-09-08

Authors

Group

University of Minnesota Hogan's Lab
Washington State University The Clowers Group

Author Contact

Hogan, Christopher
hogan108@umn.edu

Type

Dataset
Experimental Data

Abstract

Measurement of the gas-phase ion mobility of proteins provides a means to quantitatively assess the relative sizes of charged proteins. However, protein ion mobility measurements are typically singular values. Here, we apply tandem mobility analysis to low charge state protein ions (+1 and +2 ions) introduced into the gas phase by nanodroplet nebulization. We first determine protein ion mobilities in dry air and subsequently examine shifts in mobilities brought about by the clustering of vapor molecules. Tandem mobility analysis yields mobility-vapor concentration curves for each protein ion, expanding the information obtained from mobility analysis. This experimental procedure and analysis is extended to bovine serum albumin, transferrin, immunoglobulin G, and apoferritin with water, 1-butanol, and nonane. All protein ions appear to adsorb vapor molecules, with mobility “diameter” shifts of up to 6–7% at conditions just below vapor saturation. We parametrize results using κ-Köhler theory, where the term κ quantifies the extent of uptake beyond Köhler model expectations. For 1-butanol and nonane, κ decreases with increasing protein ion size, while it increases with increasing protein ion size for water. For the systems probed, the extent of mobility shift for the organic vapors is unaffected by the nebulized solution pH, while shifts with water are sensitive to pH.

Description

This data set includes the supporting data for the article, Condensable Vapor Sorption by Low Charge State Protein Ions.

Referenced by

https://doi.org/10.1021/acs.analchem.2c00357

Related to

Replaces

License

CC0 1.0 Universal
 http://creativecommons.org/publicdomain/zero/1.0/

Publisher

Collections

Data Repository for U of M (DRUM)

Funding information

US National Science Foundation Awards 2002852 and 2003042

item.page.sponsorshipfunderid

item.page.sponsorshipfundingagency

item.page.sponsorshipgrant

Previously Published Citation

Suggested citation

Lee, Jihyeon; Hogan, Christopher; Clowers, Brian. (2022). Supporting data for Condensable Vapor Sorption by Low Charge State Protein Ions. Retrieved from the Data Repository for the University of Minnesota (DRUM), https://hdl.handle.net/11299/241499.
View/Download file
File View/OpenDescriptionSize
figure1.csvRaw data for Figure 13.36 KB
figure2.csvRaw data for Figure 22.77 KB
figure3.csvRaw data for Figure 32.77 KB
figure4.csvRaw data for Figure 43.55 KB
figure5.csvRaw data for Figure 53.92 KB
figure6.csvRaw data for Figure 6751 B
Figure7.csvRaw data for Figure 7247 B
figure8.csvRaw data for Figure 81.27 KB
figure9.csvRaw data for Figure 9894 B
figure10.csvRaw data for Figure 101.09 KB
figureS2.csvData for Supplementary Figure 2978 B
readme.txtDescription of data3.24 KB

Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.