Fungal Endophytes as Priority Colonizers Initiating Wood Decomposition
Loading...
Persistent link to this item
Statistics
View StatisticsCollection period
2013
2014
2014
Date completed
2014
Date updated
Time period coverage
Geographic coverage
Source information
Journal Title
Journal ISSN
Volume Title
Title
Fungal Endophytes as Priority Colonizers Initiating Wood Decomposition
Published Date
2016-06-23
Group
Author Contact
Schilling, Jonathan
schillin@umn.edu
schillin@umn.edu
Type
Dataset
Genomics Data
Genomics Data
Abstract
Priority effects among wood decomposers have been demonstrated by manipulating fungal assembly history via inoculations in dead wood and then tracking community development using DNA sequencing. Individual wood-degrading fungi have been shown, however, to initiate decay after having colonized living trees as endophytes. To track these ‘upstream’ colonizers across the endophytesaprophyte transition, we coupled high throughput sequencing with wood physiochemical analyses in stem sections extracted from healthy birch trees (Betula papyrifera; 4-7 cm dia.). We incubated wood in microcosms, limiting communities as endophytesonly or challenging endophytes with Fomes fomentarius or Piptoporus betulinus at high exogenous inoculum potential. Initial fungal richness in birch stems averaged 143 OTUs, and decreased nearly three-fold after five months of decomposition. Although F. fomentarius successfully colonized some stem sections incubated at 25°C, decayed wood was generally dominated by saprophytic fungi that were present originally in lower abundances as endophytes. Among saprophytes, fungi in the brown rot functional guild consistently dominated, matching wood residues bearing the chemical hallmarks of brown rot. Despite this functionally redundant outcome, the taxa that rose to dominate in individual sections varied. Surprisingly, the brown rot taxa dominating wood decomposition were better known for lumber degradation rather than log decay in ground contact. Given the isolation from colonizers in our design, this redundancy of brown rot as the outcome suggests that these taxa and more generally brown rot fungi could have adapted to decompose wood where there is lower competitive pressure. Competitive avoidance would complement the diffuse depolymerization mechanisms of brown rot fungi, which are likely more prone to sugar pilfering by other organisms than the processive depolymerization mechanisms of white rot fungi. Overall, this guild-level predictability of fungal endophyte development and consequence is encouraging given the challenges of predicting wood decomposition, and it provides a base for testing these dynamics under increasing natural complexity.
Description
This repository contains the raw sequencing data for this study. The compressed file (Fungal_ITS1_MiSeq.zip) contains 82 illumia MiSeq 25 bp Read 1 FASTQ files. These files were used to generate the fungal community structure and function data in our paper, following the pipeline in the supplement File S1. The metadata of these samples were saved in the Excel file: Sample_metadata.csv
Referenced by
Song, Z., Kennedy, P. G., Liew, F. J., & Schilling, J. S. (2016). Fungal endophytes as priority colonizers initiating wood decomposition. Functional Ecology. doi: 10.1111/1365-2435.12735
http://dx.doi.org/10.1111/1365-2435.12735
http://dx.doi.org/10.1111/1365-2435.12735
Related to
Replaces
Publisher
Collections
Funding information
Conservation and the Environment grants program of The Andrew W. Mellon Foundation (New York, NY)
Doctoral Dissertation Fellowship of the University of Minnesota
Minnesota Agricultural Experiment Station funding #MIN-12-087
Doctoral Dissertation Fellowship of the University of Minnesota
Minnesota Agricultural Experiment Station funding #MIN-12-087
item.page.sponsorshipfunderid
item.page.sponsorshipfundingagency
item.page.sponsorshipgrant
Previously Published Citation
Suggested citation
Song, Zewei; Kennedy, Peter; Liew, Feng Jin; Schilling, Jonathan. (2016). Fungal Endophytes as Priority Colonizers Initiating Wood Decomposition. Retrieved from the University Digital Conservancy, http://doi.org/10.13020/D69880.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.