Effects of Active Site Inhibitors on APN-dependent Coronavirus Entry

Loading...

View/Download File

Cai_umn_0130M_18342.pdf (1.07 MB)

Persistent link to this item

https://hdl.handle.net/11299/206195

Statistics

View Statistics

Journal Title

Journal ISSN

Volume Title

Title

Effects of Active Site Inhibitors on APN-dependent Coronavirus Entry

Authors

Cai, Yijian

Published Date

2017-06

Publisher

Type

Thesis or Dissertation

Abstract

Aminopeptidase N(APN) has been shown as a receptor of several coronaviruses, such as HCoV-229E, TGEV, CCoV and FeCoV. Bestatin and Actinonin are inhibitors which can block APN enzymatic activity. These inhibitors bind to the catalytic site of APN, while viruses bind to the outer surface of APN. Here we investigate the mechanism of APN inhibition on protein-protein binding, receptor expression and coronavirus entry. We find that these chemical compounds can inhibit the protein-protein interaction between APN and Coronavirus spike; these inhibitors can also regulate APN RNA and protein expression; additionally, these compounds can inhibit the pseudovirus entry of HCoV-229E into human cells at a certain level. Additionally, coronavirus spike-treated human cells show a decrease in APN expression. This phenomenon may reveal an adaptation of cells to the different treatments and conditions. Our research may provide a new potential strategy for antiviral treatment.

Keywords

Aminopeptidase N

binding affinity

Coronavirus

inhibitor

protein expression

pseudovirus entry

Description

University of Minnesota M.S. thesis. June 2017. Major: Pharmacology. Advisor: Fang Li. 1 computer file (PDF); v, 41 pages.

Related to

Replaces

License

Collections

Master's Theses (Plan A and Professional Engineering Design Projects)

Series/Report Number

Funding information

Isbn identifier

Doi identifier

Previously Published Citation

Suggested citation

Cai, Yijian. (2017). Effects of Active Site Inhibitors on APN-dependent Coronavirus Entry. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/206195.

Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.