The Developmental Expression of Surfactant Protein-A (SP-A) in the Murine Intestinal Tract

2011-07-19
Loading...
Thumbnail Image

Persistent link to this item

Statistics
View Statistics

Journal Title

Journal ISSN

Volume Title

Title

The Developmental Expression of Surfactant Protein-A (SP-A) in the Murine Intestinal Tract

Published Date

2011-07-19

Publisher

Type

Thesis or Dissertation

Abstract

Surfactant protein-A (SP-A) plays a critical role in the innate immune system and has well characterized effects in the lung where it attenuates inflammatory responses and controls invasion of bacteria. Extra-pulmonary sources of SP-A have also been indentified: SP-A mRNA and protein have been detected in the adult gastrointestinal (GI) tract, while significant levels of SP-A protein have been detected in amniotic fluid. To date it is not clear if newborn intestinal exposure to SP-A comes from ingested amniotic fluid or from production in the newborn intestinal tract. RNA in situ hybridization with a digoxigenin-labeled anti-sense SP-A probe was used to detect cells with the SP-A mRNA message in the intestinal tract of post-natal day (PND) 3 and PND 6 mice. Immunohistochemistry in PND 7 mice was used to determine intestinal SP-A protein expression. We report positive staining for SP-A protein in cells of the lamina propria of the intestinal tract, although results were mixed as some SP-A null intestinal tissue also stained positively. RNA in situ hybridization with a digoxigenin-labeled anti-sense SP-A probe hybridized to SP-A mRNA in the lamina propria and muscularis externa of the intestinal tract, the same cells that stained positively for SP-A protein. These results indicate that SP-A is likely produced in the intestinal tract in newborn mice.

Description

Related to

Replaces

License

Series/Report Number

Funding information

Isbn identifier

Doi identifier

Previously Published Citation

Suggested citation

Theisen, Erin. (2011). The Developmental Expression of Surfactant Protein-A (SP-A) in the Murine Intestinal Tract. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/109502.

Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.