Binding interaction between PKA-C and RKIP and its mutant P74L

Olivieri, Cristina
Veglia, Gianluigi
2022-01-03
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https://doi.org/10.13020/39bz-0r13
 https://hdl.handle.net/11299/225840
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2015-07-01
2020-12-30

Date completed

2021-09-26

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Title

Binding interaction between PKA-C and RKIP and its mutant P74L

Published Date

2022-01-03

Authors

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University of Minnesota, BMBB Department, Structural Biology Division, Professor Gianluigi Veglia Lab

Author Contact

Veglia, Gianluigi
vegli001@umn.edu

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Dataset
Experimental Data
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Abstract

Kinetical and structural characterization of the interaction between the RAF kinase inhibitor (RKIP) and the cAMP-dependent protein kinase A (PKA-C) by interferometry and nuclear magnetic resonance (NMR) spectroscopy analysis. Together with the analysis performed by Dr. Marsha Rosner's laboratory (University of Chicago), these studies are part of a paper that has been sent to the PNAS journal.

Description

Raw and processed (GraphPad format) for the kinetics of binding between immobilized human PKA-C and RKIPwt obtained by biolayer interferometry analysis (BLItz - ForteBio). NMR chemical perturbation (CSP) analysis of the amide chemical shift of RKIPwt and P74L RKIP mutant in complex with ATPγN-saturatedPKA-C and CSP analysis of the amide chemical shifts of human PKA-C in complex with RKIP, under ATPγN saturating condiction.

Referenced by

Raf Kinase Inhibitory Protein regulates the cAMP-dependent protein kinase signaling pathway through a positive feedback loop" on PNAS 119(25) e2121867119
https://doi.org/10.1073/pnas.2121867119

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CC0 1.0 Universal
 http://creativecommons.org/publicdomain/zero/1.0/

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Data Repository for U of M (DRUM)

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Suggested citation

Olivieri, Cristina; Veglia, Gianluigi. (2022). Binding interaction between PKA-C and RKIP and its mutant P74L. Retrieved from the Data Repository for the University of Minnesota (DRUM), https://doi.org/10.13020/39bz-0r13.

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Description
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200717_PKA_RKIP.dataset
Raw data form BLItz of the kinetics of binding of RKIP to immobilized Human-His-tagged PKA-C (apo form)
(626.46 KB)

200721_RKIP_PKA-ATPgN.dataset
Raw data form BLItz of the kinetics of binding of RKIP to immobilized Human-His-tagged PKA-C in presence of ATP analog (PKA-C/ATPgN saturated)
(705.07 KB)

Blitz_201109.pzfx
BLItz Processed data the kinetcks of binding of RKIP to apo and ATPgN-saturated PKA-C
(2.45 MB)

Chemical_shift_perturbation.pzfx
Chemical shift perturbation(CSP) of the amide residues for RKIPwt and P74L upon the addition of PKA-C and CSP for PKA-C amide in complex with RKIPwt
(63.17 KB)

Chemical_shift_list_PKA_RKIP_P74L.xlsx
Chemical shift perturbation(CSP) of the amide residues for RKIPwt and P74L upon the addition of PKA-C and CSP for PKA-C amide in complex with RKIPwt and list file
(136.59 KB)

Readme.txt
Description of the data
(7.6 KB)

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