Binding interaction between PKA-C and RKIP and its mutant P74L
2022-01-03
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2015-07-01
2020-12-30
2020-12-30
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2021-09-26
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Title
Binding interaction between PKA-C and RKIP and its mutant P74L
Published Date
2022-01-03
Authors
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Veglia, Gianluigi
vegli001@umn.edu
vegli001@umn.edu
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Dataset
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Experimental Data
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Abstract
Kinetical and structural characterization of the interaction between the RAF kinase inhibitor (RKIP) and the cAMP-dependent protein kinase A (PKA-C) by interferometry and nuclear magnetic resonance (NMR) spectroscopy analysis. Together with the analysis performed by Dr. Marsha Rosner's laboratory (University of Chicago), these studies are part of a paper that has been sent to the PNAS journal.
Description
Raw and processed (GraphPad format) for the kinetics of binding between immobilized human PKA-C and RKIPwt obtained by biolayer interferometry analysis (BLItz - ForteBio).
NMR chemical perturbation (CSP) analysis of the amide chemical shift of RKIPwt and P74L RKIP mutant in complex with ATPγN-saturatedPKA-C and CSP analysis of the amide chemical shifts of human PKA-C in complex with RKIP, under ATPγN saturating condiction.
Referenced by
Raf Kinase Inhibitory Protein regulates the cAMP-dependent protein kinase signaling pathway through a positive feedback loop" on PNAS 119(25) e2121867119
https://doi.org/10.1073/pnas.2121867119
https://doi.org/10.1073/pnas.2121867119
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Previously Published Citation
Suggested citation
Olivieri, Cristina; Veglia, Gianluigi. (2022). Binding interaction between PKA-C and RKIP and its mutant P74L. Retrieved from the Data Repository for the University of Minnesota (DRUM), https://doi.org/10.13020/39bz-0r13.
View/Download file
| File View/Open | Description | Size |
|---|---|---|
| 200717_PKA_RKIP.dataset | Raw data form BLItz of the kinetics of binding of RKIP to immobilized Human-His-tagged PKA-C (apo form) | 626.46 KB |
| 200721_RKIP_PKA-ATPgN.dataset | Raw data form BLItz of the kinetics of binding of RKIP to immobilized Human-His-tagged PKA-C in presence of ATP analog (PKA-C/ATPgN saturated) | 705.07 KB |
| Blitz_201109.pzfx | BLItz Processed data the kinetcks of binding of RKIP to apo and ATPgN-saturated PKA-C | 2.45 MB |
| Chemical_shift_perturbation.pzfx | Chemical shift perturbation(CSP) of the amide residues for RKIPwt and P74L upon the addition of PKA-C and CSP for PKA-C amide in complex with RKIPwt | 63.17 KB |
| Chemical_shift_list_PKA_RKIP_P74L.xlsx | Chemical shift perturbation(CSP) of the amide residues for RKIPwt and P74L upon the addition of PKA-C and CSP for PKA-C amide in complex with RKIPwt and list file | 136.59 KB |
| Readme.txt | Description of the data | 7.6 KB |
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