Proton and amide chemical shift list files of wild-type cAMP-dependent protein kinase a (PKA-C) and the chimeric mutant (PKA-JC) and MATLab scripts for the chemical shift analysis

Veglia, Gianluigi
Olivieri, Cristina
Walker, Caitlin
Veliparambil Subrahmanian, Manu
2020-11-23
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https://doi.org/10.13020/7DB9-VD97
 https://hdl.handle.net/11299/217206
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July 2016
December 2018

Date completed

December 2018

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Title

Proton and amide chemical shift list files of wild-type cAMP-dependent protein kinase a (PKA-C) and the chimeric mutant (PKA-JC) and MATLab scripts for the chemical shift analysis

Published Date

2020-11-23

Authors

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University of Minnesota, BMBB Department, Structural Biology Division, Professor Gianluigi Veglia Lab

Author Contact

Veglia, Gianluigi
vegli001@umn.edu

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Abstract

Proton and amide chemical shift list files of wild-type cAMP-dependent protein kinase a (PKA-C) and the chimeric mutant (PKA-JC) and MatLab scripts used for the CHESCA and CONCISE analysis. The chemical shift lists were obtained using standard NMR experiments (1H-15N -TROSY-HSQC). The MatLab scripts were used for the CHESCA and CONCISE analysis of the amide chemical shift. These files are part of a publication on Communication biology: "Defective Internal Allosteric Network Imparts Dysfunctional ATP/Substrate Binding Cooperativity in Oncogenic Chimera of Protein Kinase A"

Description

Proton and amide chemical shift list files of wild-type cAMP-dependent protein kinase a (PKA-C) and the chimeric mutant (PKA-JC) in the apo form, ATPgN- and ATPgN/PKI-form. The list files were obtained using standard 1H-15N -TROSY-HSQC NMR experiments on uniformly 15N labeled-protein. The MatLab scripts (coded by Manu) are used for the CHEmical Shift Covariance Analysis(CHESCA) and COordiNated ChemIcal Shifts bEhavior (CONCISE) analysis of the amide chemical shift and their variants like the communtyCHESCA and the communityCONCISE.

Referenced by

Defective Internal Allosteric Network Imparts Dysfunctional ATP/Substrate Binding Cooperativity in Oncogenic Chimera of Protein Kinase A.
https://dx.doi.org/10.1038%2Fs42003-021-01819-6

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Attribution 3.0 United States
 http://creativecommons.org/licenses/by/3.0/us/

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Data Repository for U of M (DRUM)

Funding information

NIH GM 100310 to G.V

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Suggested citation

Veglia, Gianluigi; Olivieri, Cristina; Walker, Caitlin; Veliparambil Subrahmanian, Manu. (2020). Proton and amide chemical shift list files of wild-type cAMP-dependent protein kinase a (PKA-C) and the chimeric mutant (PKA-JC) and MATLab scripts for the chemical shift analysis. Retrieved from the Data Repository for the University of Minnesota (DRUM), https://doi.org/10.13020/7DB9-VD97.

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Description
Size
CHESCA.m
Chemical shift list file
(16.73 KB)

CONCISE.m
MatLab script for CONCISE analysis
(16.74 KB)

Chemical_Shifts_list_file.rar
Chemical shift list file
(49.55 KB)

Domain_and_community_CHESCA.m
Domain&Community-CHESCA
(8.33 KB)

deCONCISE.m
Domain&Community-CONCISE
(9.81 KB)

Readme.txt
Readme
(6.68 KB)

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