Between Dec 19, 2024 and Jan 2, 2025, datasets can be submitted to DRUM but will not be processed until after the break. Staff will not be available to answer email during this period, and will not be able to provide DOIs until after Jan 2. If you are in need of a DOI during this period, consider Dryad or OpenICPSR. Submission responses to the UDC may also be delayed during this time.
 

Mechanically-Induced Transition of Human Induced Pluripotent Stem Cells

Cotter, Trevor
2014-10-02
Thumbnail Image

View/Download File

Trevor Cotter magna CSE sp2014.pdf (452.81 KB)

Persistent link to this item

https://hdl.handle.net/11299/166541
Statistics
View Statistics

Journal Title

Journal ISSN

Volume Title

Title

Mechanically-Induced Transition of Human Induced Pluripotent Stem Cells

Authors

Published Date

2014-10-02

Publisher

Type

Thesis or Dissertation

Abstract

Human induced pluripotent stem cells (hiPSCs) grown in multiple culture media were altered after being subjected to a stretching protocol. hiPSC maintenance media and Gray’s media were used during the 6 day stretching protocol of 0.1 Hz with 5% circular elongation. While the final state of their development remains a mystery, qPCR using pluripotent, endoderm, and lung markers confirmed that the cells had transitioned from hiPSCs but had not been differentiated into one of these other cell lines. Further investigation must be performed to determine the cells’ new identity. Variables that could be modified in the future include cultureware coating, media used, and stretching protocols.

Keywords

Description

Related to

Replaces

License

Collections

Undergraduate Honors Theses

Series/Report Number

Funding information

Isbn identifier

Doi identifier

Previously Published Citation

Other identifiers

Suggested citation

Cotter, Trevor. (2014). Mechanically-Induced Transition of Human Induced Pluripotent Stem Cells. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/166541.

Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.