Mycobacterium tuberculosis Erdman ATCC 35801 arrayed Himar1 transposon library isolated using MtbYM rich medium.

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https://doi.org/10.13020/wt3q-0405
https://hdl.handle.net/11299/234209

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Keywords

Tn-seq

Mycobacterium tuberculosis transposon library

arrayed library mapping

Collection period

2017-04-21
2018-07-20

Date completed

2018-07-20

Date updated

Time period coverage

Geographic coverage

Source information

Journal Title

Journal ISSN

Volume Title

Title

Mycobacterium tuberculosis Erdman ATCC 35801 arrayed Himar1 transposon library isolated using MtbYM rich medium.

Published Date

2022-08-18

Authors

Tischler, Anna D

Block, Alisha

Palani, Nagendra

Brokaw, Alyssa

Zhang, Leanne

Namugenyi, Sarah

Beckman, Kenneth

Group

University of Minnesota Tischler Lab

Author Contact

Tischler, Anna D
tischler@umn.edu

Type

Dataset
Genomics Data

Abstract

We created a Himar1 transposon library in Mycobacterium tuberculosis Erdman ATCC 35801 strain containing ~8000 mutants arrayed in 80 tube racks in a 96-well format. The transposon library was isolated on MtbYM rich medium that allowed recovery of auxotrophic mutants that would not be viable on standard Middlebrook M. tuberculosis culture medium. Using orthogonal pooling and transposon sequencing (Tn-seq), we mapped the location of Tn mutants in the library. The library was split into two groups of 40 racks. For each set of 40 racks, pools were created of all mutants in each row (rows A-H), column (columns 1-12) and rack (racks 1-40 or 41-80) to generate 60 Tn-seq samples for Illumina sequencing. Two different mapping methods, the heuristic Straight Three strategy and the probabilistic Knockout Sudoku algorithm, were used to map the arrayed library with good agreement between both mapping methods. This repository contains Illumina sequencing data for all rack, column, and row pools used to map the entire 80 rack arrayed library.

Description

Illumina sequencing files for mapping the arrayed Mycobacterium tuberculosis transposon library created using MtbYM rich medium.

Referenced by

Block, Alisha M., Namugenyi, Sarah B., Palani, Nagendra P., Brokaw, Alyssa M., Zhang, Leanne, Beckman, Kenneth B., Tischler, Anna D. 2023. Mycobacterium tuberculosis Requires the Outer Membrane Lipid Phthiocerol Dimycocerosate for Starvation-Induced Antibiotic Tolerance. mSystems 8(1).
https://doi.org/10.1128/msystems.00699-22

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License

CC0 1.0 Universal
http://creativecommons.org/publicdomain/zero/1.0/

Publisher

Collections

Data Repository for U of M (DRUM)

Funding information

This work was supported by a National Institutes of Health Director’s New Innovator Award DP2AI112245 from NIAID (ADT), T90 DE02273 from NIDCR (AMB) and a University of Minnesota Doctoral Dissertation Fellowship (SNB).

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Previously Published Citation

Suggested citation

Tischler, Anna D; Block, Alisha; Palani, Nagendra; Brokaw, Alyssa; Zhang, Leanne; Namugenyi, Sarah; Beckman, Kenneth. (2022). Mycobacterium tuberculosis Erdman ATCC 35801 arrayed Himar1 transposon library isolated using MtbYM rich medium.. Retrieved from the Data Repository for the University of Minnesota (DRUM), https://doi.org/10.13020/wt3q-0405.

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