Raw data for: Biphasic Control of Cell Expansion by Auxin Coordinates Etiolated Seedling Development
2021-07-27T21:12:41Z
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2019-01-01
2021-07-26
2021-07-26
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2021-07-26
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Raw data for: Biphasic Control of Cell Expansion by Auxin Coordinates Etiolated Seedling Development
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2021-07-27T21:12:41Z
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Gray, William M
grayx051@umn.edu
grayx051@umn.edu
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Abstract
Seedling emergence is critical for food security. It requires rapid hypocotyl elongation and apical hook formation, both of which are mediated by regulated cell expansion. How these events are coordinated in etiolated seedlings is unclear. Here, we show that biphasic control of cell expansion by the phytohormone auxin underlies this process. Shortly after germination, high auxin levels restrain elongation. This provides a temporal window for apical hook formation, involving a gravity-induced auxin maximum on the eventual concave side of the hook, triggering PP2C.D1-controlled asymmetrical H+-ATPase activity, resulting in differential cell elongation. Subsequently, auxin concentrations decline acropetally and switch from restraining to promoting elongation, driving hypocotyl elongation. Our findings elucidate how differential auxin concentrations throughout the hypocotyl coordinate etiolated development, leading to successful soil emergence.
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National Institutes of Health (GM067203), USDA National Institute of Food and Agriculture (2018-67013-027503), K.C. Wong Education Foundation
Referenced by
Du et al. Science Advances 2021
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National Institutes of Health (GM067203), USDA National Institute of Food and Agriculture (2018-67013-027503), K.C. Wong Education Foundation
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| File View/Open | Description | Size |
|---|---|---|
| Readme.txt | Description of the data | 7.63 KB |
| Raw measurements.zip | Zip file containing raw measurements | 2.85 MB |
| Raw images.zip | Zip file containing raw image data | 32.02 GB |
| Raw images2.zip | Raw images acquired used in study sorted by figure (Fig S4A and Fig S4B, and movies S5, S6, S7, S8,) | 659.9 MB |
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