Proton and amide chemical shift list files of wild-type cAMP-dependent protein kinase a (PKA-C) and the chimeric mutant (PKA-JC) and MATLab scripts for the chemical shift analysis

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Persistent link to this item

https://doi.org/10.13020/7DB9-VD97
http://hdl.handle.net/11299/217206

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Keywords

Protein kinase A

Protein kinase A chimeric mutant (PKA-JC)

Chemical shift

CHESCA and CONCISE

Collection period

July 2016
December 2018

Date completed

December 2018

Date updated

Time period coverage

Geographic coverage

Source information

Journal Title

Journal ISSN

Volume Title

Title

Proton and amide chemical shift list files of wild-type cAMP-dependent protein kinase a (PKA-C) and the chimeric mutant (PKA-JC) and MATLab scripts for the chemical shift analysis

Published Date

2020-11-23

Authors

Veglia, Gianluigi

Olivieri, Cristina

Walker, Caitlin

Veliparambil Subrahmanian, Manu

Group

University of Minnesota, BMBB Department, Structural Biology Division, Professor Gianluigi Veglia Lab

Author Contact

Veglia, Gianluigi
vegli001@umn.edu

Type

Dataset
Experimental Data
Programming Software Code
Other Dataset

Abstract

Proton and amide chemical shift list files of wild-type cAMP-dependent protein kinase a (PKA-C) and the chimeric mutant (PKA-JC) and MatLab scripts used for the CHESCA and CONCISE analysis. The chemical shift lists were obtained using standard NMR experiments (1H-15N -TROSY-HSQC). The MatLab scripts were used for the CHESCA and CONCISE analysis of the amide chemical shift. These files are part of a publication on Communication biology: "Defective Internal Allosteric Network Imparts Dysfunctional ATP/Substrate Binding Cooperativity in Oncogenic Chimera of Protein Kinase A"

Description

Proton and amide chemical shift list files of wild-type cAMP-dependent protein kinase a (PKA-C) and the chimeric mutant (PKA-JC) in the apo form, ATPgN- and ATPgN/PKI-form. The list files were obtained using standard 1H-15N -TROSY-HSQC NMR experiments on uniformly 15N labeled-protein. The MatLab scripts (coded by Manu) are used for the CHEmical Shift Covariance Analysis(CHESCA) and COordiNated ChemIcal Shifts bEhavior (CONCISE) analysis of the amide chemical shift and their variants like the communtyCHESCA and the communityCONCISE.

Referenced by

Defective Internal Allosteric Network Imparts Dysfunctional ATP/Substrate Binding Cooperativity in Oncogenic Chimera of Protein Kinase A.
https://dx.doi.org/10.1038%2Fs42003-021-01819-6

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License

Attribution 3.0 United States
http://creativecommons.org/licenses/by/3.0/us/

Publisher

Collections

Data Repository for U of M (DRUM)

Funding information

NIH GM 100310 to G.V

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Previously Published Citation

Suggested citation

Veglia, Gianluigi; Olivieri, Cristina; Walker, Caitlin; Veliparambil Subrahmanian, Manu. (2020). Proton and amide chemical shift list files of wild-type cAMP-dependent protein kinase a (PKA-C) and the chimeric mutant (PKA-JC) and MATLab scripts for the chemical shift analysis. Retrieved from the University Digital Conservancy, https://doi.org/10.13020/7DB9-VD97.

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