Data for Impacts of Nanoplastics on the Viability and Riboflavin Secretion in the Model Bacteria Shewanella oneidensis
Loading...
Persistent link to this item
Statistics
View StatisticsCollection period
2019-01-05
2020-03-01
2020-03-01
Date completed
2020-06-08
Date updated
Time period coverage
Geographic coverage
Source information
Journal Title
Journal ISSN
Volume Title
Title
Data for Impacts of Nanoplastics on the Viability and Riboflavin Secretion in the Model Bacteria Shewanella oneidensis
Published Date
2020-06-30
Group
Author Contact
Type
Dataset
Experimental Data
Experimental Data
Abstract
Characterizing the impact of nanoplastics to organism health is important to understand the consequences of the environmental plastic waste problem. This article examines the impact of nano-polystyrene (nano-PS; 159±0.9 nm diameter) to ecologically relevant bacteria Shewanella oneidensis. Bacterial viability was evaluated using a growth-based assay. Riboflavin secretion is a critical cell function of S. oneidensis, serving as an electron mediator in anaerobic respiration and/or as a signaling molecule when the bacteria are under stress. Thus, changes in cellular function were monitored through riboflavin secretion in order to evaluate toxic responses that may not result in cell death. Under aerobic and anaerobic exposures (4, 8 or 12 h), the viability of the S. oneidensis was minimally changed as compared to the control, while the concentration of riboflavin secreted varied with exposure dose. In order to determine if this was a specific response to nanoplastic particles, opposed to a response to either particles or plastic more generally, we exposed the system to colloidal TiO2 nanoparticles and polystyrene and polyethylene thin films. We confirmed that riboflavin secretion trends were specific to nano-PS and not to these other materials, which showed no significant changes. We investigated the association of the nano-PS with ICP-MS using Pd that was chemically incorporated into the model nanoplastics. While 59.2% of the nano-PS were found in the non-cellular culture media, 7.0% and 6.6% was found associated with the loosely and tightly bound extracellular polymeric substance, respectively. There was significantly more nano-PS (10.9%) strongly associated with the cells. Taken together, we found that nano-PS had minimal impacts to viability but caused a significant change in the function of S. oneidensis that can be related to the nano-PS attached or in proximity to the bacterium. These trends are consistent between aerobic and anaerobic cultures, signifying that the stress response of S. oneidensis can be generalized between different environmental compartments. This work highlights that the association of nanoplastic materials with microorganisms may modify the cellular function that could ultimately be an impact to ecosystem health.
Description
Raw data files for work presented in "Impacts of Nanoplastics on the Viability and Riboflavin Secretion in the Model Bacteria Shewanella oneidensis" Frontiers in Environmental Science (2020), 8:97. DOI: 10.3389/fenvs.2020.00097
Referenced by
Fringer VS, Fawcett LP, Mitrano DM and Maurer-Jones MA (2020) Impacts of Nanoplastics on the Viability and Riboflavin Secretion in the Model Bacteria Shewanella oneidensis. Front. Environ. Sci. 8:97.
https://doi.org/10.3389/fenvs.2020.00097
https://doi.org/10.3389/fenvs.2020.00097
Related to
Replaces
item.page.isreplacedby
Publisher
Collections
Funding information
MM-J by the University of Minnesota and the Swiss National Science Foundation, Ambizione Grant number PZP002_168105 to DM
item.page.sponsorshipfunderid
item.page.sponsorshipfundingagency
item.page.sponsorshipgrant
Previously Published Citation
Other identifiers
Suggested citation
Fringer, Victoria S; Fawcett, Liam P; Mitrano, Denise M; Maurer-Jones, Melissa A. (2020). Data for Impacts of Nanoplastics on the Viability and Riboflavin Secretion in the Model Bacteria Shewanella oneidensis. Retrieved from the Data Repository for the University of Minnesota (DRUM), https://doi.org/10.13020/jka2-dt15.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.