Hard fescue (Festuca brevipila) reference transcriptome generated by PabBio Isoform sequencing

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https://doi.org/10.13020/n0mj-qv76
https://hdl.handle.net/11299/211413

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Keywords

turfgrass

Festuca brevipila Tracey

Collection period

2019-02

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Plant Growth Facility, St. Paul, MN

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Title

Hard fescue (Festuca brevipila) reference transcriptome generated by PabBio Isoform sequencing

Published Date

2020-01-31

Authors

Qiu, Yinjie

Yang, Ya

Hirsch, Cory D.

Watkins, Eric

Group

University of Minnesota Turfgrass Research Lab

Author Contact

Qiu, Yinjie
qiuxx221@umn.edu

Type

Dataset
Genomics Data

Abstract

Hard fescue (Festuca brevipila Tracey, 2n=6x=42) is a cool-season turfgrass with a fine leaf texture that performs well under low-input management. Breeding and genetics studies of F. brevipila have been limited due to the complexity of its hexaploid genome. To advance our knowledge of F. brevipila genomics, we used PacBio isoform sequencing to develop a reference transcriptome of this taxon. Here, we report a reference transcriptome generated from root, crown, leaf, and seed head tissues. We obtained 59,510 full-length transcripts of which 38,595 were non-redundant full-length transcripts, with a N50 of 2,585 bp. The longest and shortest transcripts were 11,487 and 58 bp, respectively. By using the long-read transcriptome assembly to improve the reference de novo short reads assembly we investigated the phylogenetic relationship between selected taxa that were closely related to F. brevipila. Overall, the F. brevipila Pacbio reference transcriptome improved the completeness of the short reads assembly and provided the foundation for genomics studies of this important turfgrass species.

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Referenced by

Qiu, Yinjie, et al. "Building a Reference Transcriptome for the Hexaploid Hard Fescue Turfgrass (Festuca brevipila) Using a Combination of PacBio IsoSeq and Illumina Sequencing." BioRxiv (2020). bioRxiv 2020.02.26.966952.
https://doi.org/10.1101/2020.02.26.966952

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Attribution 3.0 United States
http://creativecommons.org/licenses/by/3.0/us/

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Data Repository for U of M (DRUM)

Funding information

National Institute of Food and Agriculture, U.S. Department of Agriculture, Specialty Crop Research Initiative under award number 2017-51181-27222

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Suggested citation

Qiu, Yinjie; Yang, Ya; Hirsch, Cory D.; Watkins, Eric. (2020). Hard fescue (Festuca brevipila) reference transcriptome generated by PabBio Isoform sequencing. Retrieved from the Data Repository for the University of Minnesota (DRUM), https://doi.org/10.13020/n0mj-qv76.

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