Browsing by Subject "tumor microenvironment"
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Item Determining the Effect of Cell Culture Methods on the Polarization and Phenotype of Macrophages(2024) Lambrecht, Daniel; Chiu, Maggie; Provenzano, Paolo P.Macrophages play an important role in the regulation of cancer tumor microenvironments (TME). The specific role they play depends on their polarization, which is divided into two general phenotypes: M1, a pro-inflammatory phenotype, and M2, an anti-inflammatory phenotype known to support tumor growth. As the field of cancer research develops, there is an increased focus on understanding the role of the macrophage in the TME and how it can be manipulated to limit the growth of the tumor. Thus, it is important for researchers who are studying the macrophage’s role in the TME to know the phenotype of the macrophages that they are culturing in their research. The goal of this study was to observe the differentiation and polarization of macrophages during the standard cell culture protocol. We expected the macrophages to be fully differentiated after 7 days of culture with M-CSF and that cell passaging would result in a higher abundance of M1 polarized macrophages in culture. However, we found that macrophages are fully polarized after only 5 days of exposure to M-CSF and that passaging has no significant effect on macrophage polarization. This implies that macrophage differentiation protocols can be shortened with no loss in macrophage yield and that passaging is a suitable cell culture method for macrophages.Item REGULATION OF HYALURONAN TURNOVER IN THE DEVELOPING MAMMARY GLAND AND ITS CONTRIBUTION TO CD44 SIGNALING AND INFLAMMATION IN BREAST CANCER(2022-04) Witschen, PatriceCancer has been compared to a chronic wound unable to heal, as the balance is tipped in favor of pro-tumor inflammation. Therefore, it is important to understand how cancer cells interact with and alter their environment to ultimately support disease progression. Hyaluronan (HA) is of particular interest as it is a large glycosaminoglycan of the extracellular matrix that has anti-inflammatory effects under physiologic conditions. However, under conditions of organismal stress, an increase in HA deposition and fragmentation occurs, yet it is unknown how HA deposition impacts malignant progression. We begin by comparing HA machinery within murine mammary glands to aid in our understanding of its aberrant regulation in tumors. To our knowledge, we are the first to characterize HA deposition within the murine mammary gland across key stages of development. Additionally, our findings support a novel role for macrophages in homeostatic and aberrant HA turnover. Furthermore, we demonstrate that breast cancer cells promote cancer-associated inflammation through HA-CD44 interactions, and this axis contributes to early tumor formation. Importantly, our results are supported by data from human breast cancer cases, where increased hyaluronan synthase 2 expression significantly correlates with an inflammatory gene signature. Finally, we define a link between fibroblast growth factor receptor (FGFR)-mediated activation of HA synthesis and HA-CD44 driven macrophage recruitment during early tumorigenesis. Overall, this work establishes essential groundwork for future studies aiming to identify key targets and new therapeutic approaches for the treatment of breast cancer. Because high levels of HA deposition within many tumor types yields a poorer prognosis, our results emphasize that HA-CD44 interactions potentially have broad implications across multiple cancers.Item Using Genetics to Understand and Overcome CART Cell Resistance and Toxicities(2022-04) Cox, MichelleChimeric antigen receptor T (CART) cell therapy is an engineered cellular therapy that redirects T cells to cancer cells expressing certain antigens. CD19-directed CART cell therapy is the most advanced CART cell therapy in the clinic and is currently approved for the treatment of different B cell malignancies. However, the wider application of CART cell therapy in hematological malignancies is limited by its toxicities and lower rates of durable remission. Through translational and correlative science, large data analysis, bioinformatics, and advances in synthetic biology, we have learned that a predominant mechanism of CART cell therapy resistance is the immunosuppressive tumor microenvironment (TME). The main objective of this work was to understand how the TME impacts CART cell functions and to create inhibition-resistant CART cells utilizing genetic sequencing and synthetic biology tools. Specifically, we aimed to investigate the mechanisms by which 1) granulocyte-macrophage colony-stimulating factor (GM-CSF) and 2) leukemic extracellular vesicles (EVs) impact CART cell functions.In the first part of this work, we have discovered that GM-CSF directly impacts CART cells through modulation of their activation pathways. We have previously demonstrated that GM-CSF contributes to myeloid cell activation and to the development of toxicities after CART cell therapy. Using CRISPR/Cas9, we generated GM-CSFKO CART19 cells and demonstrated their reduced production of GM-CSF. GM-CSFKO CART19 cells demonstrated enhanced proliferation and superior anti-tumor activity in preclinical models, suggesting a direct effect of GM-CSF disruption on CART cells, independent of its known modulation of myeloid cells. To investigate the mechanism of this enhanced efficacy, we first ruled out off-target editing by performing whole exome sequencing. We then interrogated the transcriptome of GM-CSFKO CART19 cells, which showed a distinct gene expression profile suggesting alteration of activation pathways. We validated this immunophenotype on a protein level and its effect on CART cell activation and functions in vivo. In the second part of this work, we discovered a novel mechanism of resistance to CART cell therapy through their inhibition by tumor-derived extracellular vesicles. In this work, we used chronic lymphocytic leukemia (CLL) as a model to interrogate these interactions. The immunosuppressive microenvironment in CLL is well known to inhibit effector immune cells and in part may be related to the abundance of circulating EVs bearing immunomodulatory properties. We hypothesized that CLL-derived EVs contribute to CART cell dysfunction. To test this hypothesis, we first enumerated and immunophenotyped circulating EVs from platelet-free plasma in untreated patients with CLL. We determined their interaction with CART19 cells and found that CLL-derived EVs impair normal donor CART19 antigen-specific proliferation and killing. Our mechanistic studies demonstrated that CLL-derived EVs induce a state of T cell dysfunction characterized by functional, immunophenotypical, and transcriptional hallmarks of exhaustion and this dysfunction is more specific for PD-L1high EVs. In conclusion, we demonstrate that 1) CRISPR/Cas9 GM-CSF knockout in CART cells modulates their activation and enhances overall expansion and 2) leukemic EVs induce significant CART19 cell dysfunction by altering exhaustion pathways. GM-CSFKO CART19 is a novel CART cell therapy that is potentially less toxic and more effective than current CART19. The knowledge that leukemic EVs induce CART cell dysfunction paves the way for future studies of EV phenotype and cargo, which can ultimately lead to new strategies to predict outcomes and implement individualized CART cell therapy.