Using Genetics to Understand and Overcome CART Cell Resistance and Toxicities
2022-04
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Using Genetics to Understand and Overcome CART Cell Resistance and Toxicities
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2022-04
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Chimeric antigen receptor T (CART) cell therapy is an engineered cellular therapy that redirects T cells to cancer cells expressing certain antigens. CD19-directed CART cell therapy is the most advanced CART cell therapy in the clinic and is currently approved for the treatment of different B cell malignancies. However, the wider application of CART cell therapy in hematological malignancies is limited by its toxicities and lower rates of durable remission. Through translational and correlative science, large data analysis, bioinformatics, and advances in synthetic biology, we have learned that a predominant mechanism of CART cell therapy resistance is the immunosuppressive tumor microenvironment (TME). The main objective of this work was to understand how the TME impacts CART cell functions and to create inhibition-resistant CART cells utilizing genetic sequencing and synthetic biology tools. Specifically, we aimed to investigate the mechanisms by which 1) granulocyte-macrophage colony-stimulating factor (GM-CSF) and 2) leukemic extracellular vesicles (EVs) impact CART cell functions.In the first part of this work, we have discovered that GM-CSF directly impacts CART cells through modulation of their activation pathways. We have previously demonstrated that GM-CSF contributes to myeloid cell activation and to the development of toxicities after CART cell therapy. Using CRISPR/Cas9, we generated GM-CSFKO CART19 cells and demonstrated their reduced production of GM-CSF. GM-CSFKO CART19 cells demonstrated enhanced proliferation and superior anti-tumor activity in preclinical models, suggesting a direct effect of GM-CSF disruption on CART cells, independent of its known modulation of myeloid cells. To investigate the mechanism of this enhanced efficacy, we first ruled out off-target editing by performing whole exome sequencing. We then interrogated the transcriptome of GM-CSFKO CART19 cells, which showed a distinct gene expression profile suggesting alteration of activation pathways. We validated this immunophenotype on a protein level and its effect on CART cell activation and functions in vivo.
In the second part of this work, we discovered a novel mechanism of resistance to CART cell therapy through their inhibition by tumor-derived extracellular vesicles. In this work, we used chronic lymphocytic leukemia (CLL) as a model to interrogate these interactions. The immunosuppressive microenvironment in CLL is well known to inhibit effector immune cells and in part may be related to the abundance of circulating EVs bearing immunomodulatory properties. We hypothesized that CLL-derived EVs contribute to CART cell dysfunction. To test this hypothesis, we first enumerated and immunophenotyped circulating EVs from platelet-free plasma in untreated patients with CLL. We determined their interaction with CART19 cells and found that CLL-derived EVs impair normal donor CART19 antigen-specific proliferation and killing. Our mechanistic studies demonstrated that CLL-derived EVs induce a state of T cell dysfunction characterized by functional, immunophenotypical, and transcriptional hallmarks of exhaustion and this dysfunction is more specific for PD-L1high EVs.
In conclusion, we demonstrate that 1) CRISPR/Cas9 GM-CSF knockout in CART cells modulates their activation and enhances overall expansion and 2) leukemic EVs induce significant CART19 cell dysfunction by altering exhaustion pathways. GM-CSFKO CART19 is a novel CART cell therapy that is potentially less toxic and more effective than current CART19. The knowledge that leukemic EVs induce CART cell dysfunction paves the way for future studies of EV phenotype and cargo, which can ultimately lead to new strategies to predict outcomes and implement individualized CART cell therapy.
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University of Minnesota Ph.D. dissertation. April 2022. Major: Biomedical Informatics and Computational Biology. Advisors: Chad Myers, Saad Kenderian. 1 computer file (PDF); xii, 132 pages.
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Cox, Michelle. (2022). Using Genetics to Understand and Overcome CART Cell Resistance and Toxicities. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/241374.
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