Browsing by Subject "Protein kinase A chimeric mutant (PKA-JC)"
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Item Proton and amide chemical shift list files of wild-type cAMP-dependent protein kinase a (PKA-C) and the chimeric mutant (PKA-JC) and MATLab scripts for the chemical shift analysis(2020-11-23) Veglia, Gianluigi; Olivieri, Cristina; Walker, Caitlin; Veliparambil Subrahmanian, Manu; vegli001@umn.edu; Veglia, Gianluigi; University of Minnesota, BMBB Department, Structural Biology Division, Professor Gianluigi Veglia LabProton and amide chemical shift list files of wild-type cAMP-dependent protein kinase a (PKA-C) and the chimeric mutant (PKA-JC) and MatLab scripts used for the CHESCA and CONCISE analysis. The chemical shift lists were obtained using standard NMR experiments (1H-15N -TROSY-HSQC). The MatLab scripts were used for the CHESCA and CONCISE analysis of the amide chemical shift. These files are part of a publication on Communication biology: "Defective Internal Allosteric Network Imparts Dysfunctional ATP/Substrate Binding Cooperativity in Oncogenic Chimera of Protein Kinase A"Item Steady-state coupled enzyme assays using standard peptides of cAMP-dependent protein kinase a catalytic subunit (PKA-C) and its chimeric mutant (PKA-CJ)(2020-11-23) Olivieri, Cristina; vegli001@umn.edu; Veglia, GianluigiSteady-state coupled enzyme assays using standard peptide substrates (Kempite, CREB, and KSR1) of cAMP-dependent protein kinase A (PKA-C) and its chimeric mutant (PKA-JC)