Browsing by Subject "Molecular biology"

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    Comparative genomics of Ornithobacterium rhinotracheale and determination of strain-specific pathogenicity and virulence
    (2021-09) Smith, Emily
    The subsequent chapters of this dissertation will address many of the current knowledge gaps surrounding ORT. First, comparative genomics of clinical ORT isolates from several US commercial turkey producers will highlight the genetic similarities and differences between currently circulating ORT strains. Second, a study comparing these clinical isolates to commensal isolates of ORT will reveal whether there are genetic differences between clinical and commensal isolates. Finally, a series of challenge studies will determine if clinical ORT strains that differ genomically result in differences clinically, and if controlled exposure is effective in preventing negative outcomes associated with ORT.
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    The Utilization of Algicidal Bacteria for Improved Lipid Extractions and Insights into Neutral Lipid Production in a Wax Ester Accumulating Bacterium
    (2013-08) Lenneman, Eric M.
    Based on finite and diminishing quantities of available fossil fuels and the increased demand by a growing population, the identification and production of biologically derived fuels and alternatives to petroleum based compounds has become increasingly important. Biological fuels hold promise from both an economical and environmental standpoint. Microorganisms may hold the key to producing these compounds as many algae and bacteria have been found to produce high quantities of lipids and other bioproducts similar to those obtained from fossil fuels. The purpose of these studies was to evaluate the potential application of algae-degrading bacteria to aid in lipid extractions from the microalgae Neochloris oleoabundans and Dunaliella tertiolecta. Separate studies evaluated the wax ester biosynthetic pathway in the lipid accumulating bacterium Marinobacter aquaeolei VT8 through the analysis of transcriptional levels within wild-type cells, and finally these studies were complimented by gene deletion efforts for specific enzymes within this biosynthetic pathway.