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Browsing by Subject "Lillehei Heart Institute"

Now showing 1 - 2 of 2
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    ER71 Transcriptionally Activates Brachyury: Study of Molecular Mechanisms Involved in Gene Regulation
    (2010-04-21) Eck, Leslie
    Congenital cardiac malformation is the most frequent birth defect which contributes to advanced heart failure in the pediatric and adult population. An enhanced understanding of the transcriptional networks that direct cardiac progenitors during heart development will have important therapeutic applications for the treatment of congenital heart disease. Furthermore, a number of parallel transcriptional pathways or networks have been proposed for the generation and regeneration of tissues such as the heart. For these reasons we predict that the definition of the transcriptional regulatory mechanisms of cardiac progenitor cells in the developing heart will enhance our understanding of cardiogenesis, congenital heart disease and myocardial regeneration. Previously, using transcriptome and RT-PCR analysis we found that ER71 was dysregulated in Nkx2-5 null embryos at both E8.0 and E9.5 in comparison to their WT littermates. This data established that ER71 is a direct downstream target of the homoedomain protein Nkx2-5. Here, we will focus on transcriptional regulation of cardiogenesis by Nkx 2.5, Etsrp71, and Brachyury (T protein).
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    A fast and efficient method to generate contracting embryoid bodies from murine iPs cells
    (2012-04-18) Chen, Denise
    This project aimed to test and refine a method of differentiating iPs cells into contracting embryoid bodies. Embryoid bodies (EBs) are spheroid clumps of pluripotent cells that can differentiate into various types of cells5. Spontaneous contraction of EBs indicates development into cardiomyocytes. The ability to differentiate cardiomyocytes from iPs cells can then aid in the process of cardiomyocyte regeneration for individuals who have suffered significant and damaging cardiac events. Our research aimed to develop a method of quickly and efficiently differentiating murine iPs cells into contracting EBs. Our protocol yielded areas of spontaneously contracting proportions of EBs within six days, and the peak proportion of contracting EBs was observed within nine to ten days. Further research into this topic may look into the role of norepinephrine in successfully developing EBs, or the applicability of this method of differentiation on human iPs cells.