Browsing by Subject "Fusion Protein"
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Item TEV Protease Cleavage of MBP and His Affinity Tags in de novo Fusion Proteins(2023-07) Soltau, Alexander D.; Malisetti, Nithya; Winslow, Peter J; Seelig, BurckhardPolyhistidine (His) and Maltose Binding Protein (MBP) tagged proteins were successfully expressed in Escherichia coli. TEV protease cleavage and MBP-free cloning were pursued as strategies for obtaining pure, MBP-free protein variants for further research. A protocol for TEV cleavage was developed, and methods of subsequent purification or alternative cloning were explored.