Between Dec 19, 2024 and Jan 2, 2025, datasets can be submitted to DRUM but will not be processed until after the break. Staff will not be available to answer email during this period, and will not be able to provide DOIs until after Jan 2. If you are in need of a DOI during this period, consider Dryad or OpenICPSR. Submission responses to the UDC may also be delayed during this time.
 

TEV Protease Cleavage of MBP and His Affinity Tags in de novo Fusion Proteins

Soltau, Alexander D.
Malisetti, Nithya
Winslow, Peter J
Seelig, Burckhard
2023-07
Thumbnail Image

View/Download File

Research Poster (447.71 KB)

Persistent link to this item

https://hdl.handle.net/11299/256042
Statistics
View Statistics

Journal Title

Journal ISSN

Volume Title

Title

TEV Protease Cleavage of MBP and His Affinity Tags in de novo Fusion Proteins

Authors

Published Date

2023-07

Publisher

Type

Presentation

Abstract

Polyhistidine (His) and Maltose Binding Protein (MBP) tagged proteins were successfully expressed in Escherichia coli. TEV protease cleavage and MBP-free cloning were pursued as strategies for obtaining pure, MBP-free protein variants for further research. A protocol for TEV cleavage was developed, and methods of subsequent purification or alternative cloning were explored.

Keywords

Description

UROP Project conducted in the Seelig Lab under the direction of Burckhard Seelig. Polyhistidine (His) and Maltose Binding Protein (MBP) tagged proteins were successfully expressed in Escherichia coli. TEV protease cleavage and MBP-free cloning were pursued as strategies for obtaining pure, MBP-free protein variants for further research. A protocol for TEV cleavage was developed, and methods of subsequent purification or alternative cloning were explored.

Related to

Replaces

License

Collections

UMTC Undergraduate Research Presentations and Papers (UROP)

Series/Report Number

Funding information

This research was supported by the Undergraduate Research Opportunities Program (UROP) and by NASA under award 80NSSC21K0595.

Isbn identifier

Doi identifier

Previously Published Citation

Other identifiers

Suggested citation

Soltau, Alexander D.; Malisetti, Nithya; Winslow, Peter J; Seelig, Burckhard. (2023). TEV Protease Cleavage of MBP and His Affinity Tags in de novo Fusion Proteins. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/256042.

Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.