Department of Plant Pathology

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https://conservancy.umn.edu/handle/11299/923

The Department of Plant Pathology at the University of Minnesota has a strong research and teaching emphasis in disease resistance, molecular genetics and genomics, control of diseases caused by biotic pathogens, wood deterioration, effect of air pollution on plants, biological control, ecology and evolution of plant-associated microbes, and in the physiology and molecular biology of plant-microbe interactions. The department also has a strong outreach emphasis through the extension service, plant disease clinic, and other avenues.

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Browsing Department of Plant Pathology by Subject "allelism testing"

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    Identification and mapping of a leaf rust resistance gene in barley line Q21861
    (Genome, 1997) Steffenson, Brian; Borovkova, I.G.; Jin, Y.; Kilian, A.; Blake, T.K.; Kleinhofs, A.
    Barley line Q21861 possesses an incompletely dominant gene (RphQ) for resistance to leaf rust caused by Puccinia hordei. To investigate the allelic and linkage relations between RphQ and other known Rph genes, F2 populations from crosses between Q21861 and donors of Rph1 to Rph14 (except for Rph8) were evaluated for leaf rust reaction at the seedling stage. Results indicate that RphQ is either allelic with or closely linked to the Rph2 locus. A doubled haploid population derived from a cross between Q21861 and SM89010 (a leaf rust susceptible line) was used for molecular mapping of the resistance locus. Bulked segregant analysis was used to identify markers linked to RphQ, using random amplified polymorphic DNAs (RAPDs), restriction fragment length polymorphisms (RFLPs), and sequence tagged sites (STSs). Of 600 decamer primers screened, amplified fragments generated by 9 primers were found to be linked to the RphQ locus; however, only 4 of them were within 10 cM of the target. The RphQ locus was mapped to the centromeric region of chromosome 7, with a linkage distance of 3.5 cM from the RFLP marker CDO749. Rrn2, an RFLP clone from the ribosomal RNA intergenic spacer region, was found to be very closely linked with RphQ, based on bulked segregant analysis. An STS marker, ITS1, derived from Rrn2, was also closely linked (1.6 cM) to RphQ.