Browsing by Author "Li, Lin"

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    Phenotypic and genotypic data of barley Gen10 population to study a pericentromeric region on chromosome 6H
    (2021-06-01) Yin, Lu; Huang, Yadong; Sallam, Ahmad; Heinen, Shane; Li, Lin; Beaubien, Karen; Dill-Macky, Ruth; Dong, Yanhong; Steffenson, Brian; Smith, Kevin; Muehlbauer, Gary; smith376@umn.edu; Smith, Kevin; University of Minnesota Barley Breeding and Genetics (Smith Lab)
    The phenotypic and genotypic data of the Gen10 population used to study a genetic region on chromosome 6H of barley associated with multiple traits. Gen10 is a population of ~100 Chevron-derived recombinant near-isogenic lines that contained the Fusarisum head blight/kernel discoloration resistance locus from Chevron, a landrace, in the genetic background of Lacey, an elite malting barley variety.
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    Supporting data for Development of a multi-parent population for genetic mapping and allele discovery in six-row barley
    (2019-08-12) Hemshrot, Alex; Poets, Ana M; Tyagi, Priyanka; Lei, Li; Carter, Corey; Hirsch, Candice N; Li, Lin; Brown-Guedira, Gina; Morrell, Peter L; Muehlbauer, Gary J; Smith, Kevin P; llei@umn.edu; Lei, Li; University of Minnesota Department of Plant and Microbial Biology; HuaZhong Agricultural University Department of Genetics, College of Plant Science and Technology; USDA Eastern Regional Small Grains Genotyping Laboratory; University of Minnesota Department of Agronomy & Plant Genetics
    Germplasm collections hold valuable allelic diversity for crop improvement and genetic mapping of complex traits. To gain access to the genetic diversity within the USDA National Small Grain Collection (NSGC), we developed the Barley Recombinant Inbred Diverse Germplasm Population (BRIDG6), a six-row spring barley multi-parent population (MPP) with 88 cultivated accessions ranging from landrace to cultivars crossed to a common parent (Rasmusson). The parents were randomly selected from a core subset of the NSGC that represents the genetic diversity of landrace and breeding accessions. In total, we generated 6,160 F5 recombinant inbred lines (RILs) with an average of 69 and a range of 37-168 RILs per family genotyped with 7,773 SNPs. The number of segregating SNPs per family range from 956 to 6,775, with an average of 3,889 SNPs per family. Using BRIDG6, we detected 23 QTL contributing to flowering time. Five QTL were within five megabase pairs of previously described flowering time genes. For the major QTL detected near HvPpd-H1, a flowering time gene that affects photoperiod, we observed both positive and negative allele effects ranging from +4 to –3 days relative to Rasmusson among the 79 families segregating for the SNP. Haplotype-based analysis of HvPpd-H1 identified private alleles to families of Asian origin conferring both positive and negative effects, providing the first observation of flowering time-related alleles private to Asian accessions. We evaluate several subsampling strategies to determine their effect on the power of QTL detection and found that for flowering time in barley, a sample size larger than 50 families or 3,000 individuals results in the highest QTL detection. This MPP will be useful for uncovering large and small effect QTL for traits of interest and identifying and utilizing valuable alleles from the NSGC for barley improvement.
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    Use of Fly Ash for Reconstruction of Bituminous Roads
    (Minnesota Department of Transportation, 2009-08) Benson, Craig; Edil, Tuncer; Bloom, Paul; Ebrahimi, Ali; Kootstra, Brian; Li, Lin
    Recycling part or all of the pavement materials in an existing road during reconstruction is an attractive construction alternative. When reconstructing roads surfaced with hot mix asphalt (HMA), the HMA, underlying base, and a portion of the existing subgrade often are pulverized to form a new base material referred to as recycled pavement material (RPM). Compacted RPM is overlain with a new HMA layer to create a reconstructed or rehabilitated pavement. This process is often referred to as full-depth reclamation. Similarly, when an unpaved road with a gravel surface is upgraded to a paved road, the existing road surface gravel (RSG) is blended and compacted to form a new base layer that is overlain with an HMA surface. Recycling pavement and road materials in this manner is both cost effective and environmentally friendly. However, recycled base materials may contain asphalt binder, fines, and/or other deleterious materials that can adversely affect strength and stiffness. To address this issue, chemical stabilizing agents can be blended with RPM or RSG. Use of industrial material resources for stabilization (e.g., cementitious coal fly ash) is particularly attractive in the context of sustainability. The purpose of this study was to develop a practical method to design local roadways using stabilized RPM or SRSG as the base layer and Class C fly ash as the stabilizing agent. The design method was developed in the context of the “gravel equivalency” (GE) design methodology employed for local roads in Minnesota.