Browsing by Author "Franzen-Klein, Dana"
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Item Evaluation of Neurobehavioral Abnormalities and Immunotoxicity after Oral Imidacloprid Exposure in Domestic Chickens (Gallus gallus domesticus)(2019-05) Franzen-Klein, DanaNeonicotinoid pesticides may have negative effects on non-target species at environmentally plausible exposure doses. The objective of the present study was to quantify neurobehavioral abnormalities and immunotoxicity due to oral imidacloprid exposure in birds. Domestic white leghorn chickens (Gallus gallus domesticus; n=120) were exposed to imidacloprid by gavage once daily for 7 consecutive days at 0, 0.03, 0.34, 3.42, 10.25, and 15.50 mg/kg. The severity and duration of neurobehavioral abnormalities were recorded, and immune function was assessed with 7 standard functional assays. Immunotoxicity was not detected. Temporary neurobehavioral abnormalities were observed in a dose-dependent manner, including generalized whole-body muscle tremors, ataxia, and depressed mentation ranging from mild depression to a complete lack of response to external stimulation. The effect dose value for the presence of any neurobehavioral abnormalities in 50% of the test group (ED50) was 4.63 mg/kg/day. The ED50 for an adjusted score that included both the severity and duration of neurobehavioral abnormalities was 11.27 mg/kg/day. The no observed adverse effect level (NOAEL) and lowest observed effect level (LOEL) were 3.42 mg/kg/day, and the lowest observed adverse effect level (LOAEL) was 10.25 mg/kg/day. While immunotoxicity was not demonstrated in the present study, it cannot be ruled out. The observed neurobehavioral abnormalities were severe at the higher doses and may impair survival of free-living gallinaceous birds.Item Evaluation of neurobehavioral abnormalities and immunotoxicity in response to oral imidacloprid exposure in domestic chickens (Gallus gallus domesticus)(Journal of Toxicology and Environmental Health, Part A, 2020) Franzen-Klein, Dana; Jankowski, Mark; Roy, Charlotte L.; Nguyen-Phuc, Hoa; Chen, Da; Neuman-Lee, Lori; Redig, Patrick; Ponder, JulieDomestic chickens (Gallus gallus domesticus) were exposed to imidacloprid by gavage once daily for 7 consecutive days at 0, 0.03, 0.34, 3.42, 10.25, and 15.5 mg/kg/day (n = 20 per group; 5 6-week-old males, 5 6-week-old females, 5 9-week-old males, and 5 9-week-old females). The severity and duration of neurobehavioral abnormalities were recorded. Components of the innate and adaptive immune system were assessed with 7 standard functional assays. Temporary neurobehavioral abnormalities were observed in a dose-dependent manner, including muscle tremors, ataxia, and depressed mentation. Based upon mean clinical severity scores, the no observed adverse effect level (NOAEL) was 3.42 mg/kg/day, and the lowest observed adverse effect level (LOAEL) was 10.25 mg/kg/ day. The effective dose value for the presence of any neurobehavioral abnormalities in 50% of the test group (ED50) was 4.62 ± 0.98 mg/kg/day. The ED50 for an adjusted score that included both severity and duration of neurobehavioral abnormalities was 11.24 ± 9.33 mg/kg/day. These ED50 values are equivalent to a 1 kg bird ingesting 29 or 70 imidacloprid treated soybean seeds respectively. Immunotoxicity was not documented, possible causes include the assays were insensitive, relevant immune functions were not examined, or imidacloprid is not immunotoxic at this dosing schedule in this species. Neurobehavioral abnormalities were a more sensitive indicator of the sublethal effects of imidacloprid than immunotoxicity.Item Surveillance for highly pathogenic avian influenza A (H5N1) in a raptor rehabilitation center — 2022(2024-03-11) Hall, Victoria L; Cardona, Carol; Mendoza, Kristelle; Torchetti, Mia; Lantz, Kristina; Bueno, Irene; Franzen-Klein, Dana; hall2112@umn.edu; Hall, Victoria L; University of Minnesota The Raptor CenterAn ongoing, severe outbreak of highly pathogenic avian influenza virus (HPAI) A H5N1 clade 2.3.4.4b has been circulating in wild and domestic bird populations throughout the world, reaching North America in 2021. This HPAI outbreak has exhibited unique characteristics when compared to previous outbreaks. The global distribution of disease, prolonged duration, extensive number of species and individual wild birds affected, and the large impact on the global poultry industry have all exceeded historical impacts of previous outbreaks in North America. In this study, we describe the results of HPAI surveillance conducted at The Raptor Center, a wildlife rehabilitation hospital at University of Minnesota (Saint Paul, MN, U.S.A.), from March 28th – December 31, 2022. All wild raptors admitted to the facility were tested for avian influenza viruses using polymerase chain reaction (PCR) testing. All non-negative samples were submitted to the United States Department of Agriculture (USDA) Animal and Plant Health Inspection Service (APHIS) National Veterinary Services Laboratories for confirmatory HPAI testing and genetic sequencing. During the study period, 996 individual birds representing 20 different species were tested for avian influenza, and 213 birds were confirmed HPAI positive. Highly pathogenic avian influenza surveillance conducted at The Raptor Center contributed 75% of the HPAI positive raptor detections within the state of Minnesota, located within the Mississippi flyway, significantly augmenting state wildlife surveillance efforts. The viral genotypes observed in birds sampled at The Raptor Center were representative of what was seen in wild bird surveillance within the Mississippi flyway during the same time frame. Wildlife rehabilitation centers provide an opportune situation to augment disease surveillance at the human, wildlife and domestic animal interface during ongoing infectious disease outbreaks.