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Browsing by Author "Cotter, Trevor"

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    Mechanically-Induced Transition of Human Induced Pluripotent Stem Cells
    (2014-10-02) Cotter, Trevor;
    Human induced pluripotent stem cells (hiPSCs) grown in multiple culture media were altered after being subjected to a stretching protocol. hiPSC maintenance media and Gray’s media were used during the 6 day stretching protocol of 0.1 Hz with 5% circular elongation. While the final state of their development remains a mystery, qPCR using pluripotent, endoderm, and lung markers confirmed that the cells had transitioned from hiPSCs but had not been differentiated into one of these other cell lines. Further investigation must be performed to determine the cells’ new identity. Variables that could be modified in the future include cultureware coating, media used, and stretching protocols.
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    Synthesizing BACE1 Proteins to Investigate the Pathogenesis of Alzheimer’s Disease
    (2011-10-17) Cotter, Trevor
    The purpose of my project was to help design recombinant plasmids that would be used to create BACE1 proteins, which are involved in the pathogenesis of Alzheimer’s Disease (AD). BACE1 cleaves the amyloid precursor protein (APP) which is found in the cell membrane. Then γ-secretase cuts the resulting peptide, forming amyloid-β (Aβ) peptides. These Aβ peptides aggregate in the brain and cause plaques that lead to AD.