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Browsing by Author "Costa, Kyle"

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    Data for Formate dependent heterodisulfide reduction in a Methanomicrobiales archaeon
    (2020-12-01) Abdul Halim, Mohd Farid; Day, Leslie; Costa, Kyle; kcosta@umn.edu; Costa, Kyle; Costa Lab
    Hydrogenotrophic methanogens produce CH4 using H2 as electron donor to reduce CO2. In the absence of H2, many are able to use formate or alcohols as alternate electron donors. Methanogens from the order Methanomicrobiales are capable of growth with H2, but many lack genes encoding hydrogenases that are typically found in other hydrogenotrophic methanogens. In an effort to better understand electron flow in methanogens from the Methanomicrobiales, we undertook a genetic and biochemical study of heterodisulfide reductase (Hdr) in Methanoculleus thermophilus. Hdr catalyzes an essential reaction by coupling the first and last steps of methanogenesis through flavin-based electron bifurcation. Hdr from M. thermophilus co-purified with formate dehydrogenase (Fdh) and only displayed activity when formate was supplied as an electron donor. We found no evidence of an Hdr associated hydrogenase, and H2 could not function as an electron donor, even with Hdr purified from cells grown on H2. We found that cells catalyze a formate hydrogenlyase activity that is likely essential for generating the formate needed for the Hdr reaction. Together, these results highlight the importance of formate as an electron donor for methanogenesis and suggest the ability to use formate is closely integrated into the methanogenic pathway in organisms from the order Methanomicrobiales.
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    Supporting data for The fluorescence-activating and absorption-shifting tag (FAST) enables live-cell fluorescence imaging of Methanococcus maripaludis
    (2022-05-06) Hernandez, Eric; Costa, Kyle; kcosta@umn.edu; Costa, Kyle; University of Minnesota Costa Lab
    Microscopy images of Methanococcus maripaludis cells expressing FAST1 tagged to different genes involved in methanogenesis. Images were used for quantification of protein expression in live cells.