Browsing by Author "Aparicio, Conrado"

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    16S RNA data for biofilm in contact with antimicrobial peptide coatings
    (2020-04-23) Aparicio, Conrado; Moussa, Dina G.; apari003@umn.edu; Aparicio, Conrado; Minnesota Dental Research Center for Biomaterials and Biomechanics
    Dual-indexed 16S rRNA gene amplicon sequencing using the V3-V4 region on the Illumina MiSeq platform 300PE for triplicate samples of biofilm from stocks of oral plaque sample grown on hydroxyapaptite discs without (CTRL) and with antimicrobial peptide (1018, DJK2, D-GL13K) coatings, treated and non-treated with PMA. This data was collected as part of the NIDCR funded project R01-DE to determine the effects of antimicrobial peptides in the microbiome of biofilms from oral plaque stocks to prevent degradation of dental restorations. The data was generated at the University of Minnesota Genomics Center and analyzed at the University of Minnesota Informatics Institute. The data is released to be of public access following submission of a manuscript presenting and analyzing this data.
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    Data for Utilizing a degradation prediction pathway system to understand how a novel methacrylate derivative polymer with flipped external ester groups retains physico-mechanical properties following esterase exposure
    (2024-08-22) Kumar, Dhiraj; Ghose, Debarati; Mutreja, Isha; Bolskar, Robert; Aparicio, Conrado; Jones, Robert S; rsjones@umn.edu; Jones, Robert S; B-A-M Lab
    The region of failure for current methacrylates (i.e. derivatives of acrylates) are ester bond linkages that hydrolyze in the presence of salivary and bacterial esterases that break the polymer network backbone. This effect decreases the mechanical properties of methacrylate-based materials. The ethylene glycol dimethacrylate (EGDMA) or novel ethylene glycol ethyl methacrylate (EGEMA) discs were prepared using 40μL of the curing mixture containing photo/co-initiators for 40second in a PTFE mold at 1000mW/cm2. The degree of conversion was used as a quality control measure for the prepared disks, followed by physical, mechanical, and chemical characterization of discs properties before and after cholesterol esterase treatment. After 9 weeks of standardized cholesterol esterase (CEase) exposure, EGDMA discs showed exponential loss of material (p=0.0296), strength (p=0.0014) and increased water sorption (p=0.0002) compared to EGEMA discs. We integrated a degradation prediction pathway system to LC/MS and GC/MS analyses to elucidate the degradation by-products of both EGEMA and EGDMA polymers. GC/MS analysis demonstrated that the esterase catalysis was directed to central polymer backbone breakage, producing ethylene glycol, for EGDMA, and to side chain breakage, producing ethanol, for EGEMA. The flipped external ester group linkage design is attributed to EGEMA showing higher resistance to esterase biodegradation and changes in mechanical and physical properties than EGDMA. EGEMA is a potential substitute for common macromer diluents, such as EGDMA, based on its resistance to biodegradation effects. This work inspires the flipped external group design to be applied to analogs of current larger, hydrophobic strength bearing macromers used in future dental material formulations. The data in this record supports the figures in the related manuscript.