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University of Minnesota Libraries Response to "Defense Federal Acquisition Regulation Supplement: Public Access to Results of Federally Funded Research" (2024)
(2024-10-29) University Libraries; Hunt, Shanda; Farrell, Shannon L; Marsolek, Wanda; Langham-Putrow, Allison
Role of the cGAS-STING Signaling Pathway in 4-Hydroxynonenal-Induced Cellular Senescence
(2024) Fish, Shayla;
The cGAS-STING signaling pathway is responsible for recognizing cytosolic doublestranded
DNA and initiating an inflammatory response as part of the innate immune system. This
pathway has been correlated with the development of cellular senescence, a state of irreversible
cell cycle arrest. Senescent cells accumulate with age and a senescent phenotype is associated
with chronic inflammation and numerous age-related diseases. There is a lack of research
connecting the cGAS-STING pathway to cellular senescence initiated by endogenous inducers of
senescence. Using the endogenously produced reactive lipid aldehyde 4-Hydroxynonenal (4-
HNE) to induce senescence, various small molecule inhibitors to components of the cGASSTING
pathway were used to further examine this connection. Murine stromal vascular fraction
cells and IMR90 cells, an immortalized human lung fibroblast cell line, were cultured and treated
with 4-HNE in addition with two different small-molecule inhibitors of cGAS-STING pathway
components. Using immunoblotting and quantitative real-time PCR techniques, protein and
mRNA levels were analyzed to assess cGAS-STING pathway activation and the presence of a
senescent phenotype with and without inhibitor presence. The results show upregulation of
numerous protein and mRNA markers suggesting cGAS-STING pathway activation, as well as
evidence of a senescent phenotype in cells treated with 4-HNE. However, both small molecule
inhibitors showed inconsistent results in preventing a senescent phenotype. The results suggest
the possibility of a mechanistic correlation between the cGAS-STING pathway and cellular
senescence, however future work is required to further examine this relationship in the context of
4-HNE.
What’s New with Youth in 2023 The State of Adolescent Sexual Health in Minnesota Webinar
(2023) Healthy Youth Development - Prevention Research Center
2023 Winona County Adolescent Sexual Health Report
(2023) Healthy Youth Development - Prevention Research Center
2023 Yellow Medicine County Adolescent Sexual Health Report
(2023) Healthy Youth Development - Prevention Research Center