EPR Analysis of Myosin Structural Dynamics

No Thumbnail Available

Persistent link to this item

View Statistics

Journal Title

Journal ISSN

Volume Title


EPR Analysis of Myosin Structural Dynamics

Published Date






The structural dynamics of myosin during muscle contraction can be discerned in situ through site directed spin labeling of myosin and electron paramagnetic resonance (EPR) spectroscopy. To achieve in situ measurements, spin labeled myosin regulatory light chain (RLC) is exchanged for endogenous RLC in rabbit psoas fiber bundles. In order to ensure the structural integrity of exchanged muscle fibers, functional measurements must be done before and after exchange. After verifying function after RLC exchange, we can use EPR to measure the orientational dynamics of the RLC in a variety of states during muscle contraction.


Additional contributors: Ryan Mello; Amanda Laden; David D. Thomas (faculty mentor).

Related to



Series/Report Number

Funding information

This research was supported by the Undergraduate Research Opportunities Program (UROP).

Isbn identifier

Doi identifier

Previously Published Citation

Suggested citation

Harris, Robert. (2009). EPR Analysis of Myosin Structural Dynamics. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/58250.

Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.