Automation of Stem Cell Protocols
2019-12
Loading...
Persistent link to this item
Statistics
View StatisticsJournal Title
Journal ISSN
Volume Title
Title
Automation of Stem Cell Protocols
Authors
Published Date
2019-12
Publisher
Type
Thesis or Dissertation
Abstract
Human induced pluripotent stem cells (hiPSCs) have become a vital resource for researchers and industry due to their differentiation capacity, as well as providing access to the cell phenotypes and genotypes from any individual donor. Despite improvements in stem cell technology, maintaining iPS cell lines still requires a significant amount of time and technical skill from cell culture technicians. Such steps include consistent media changes, cell counting and confluence analyses, cell passaging, cryopreservation, and subsequent thawing and plating of those cells. For this research, these processes have been transitioned onto an automated cell culturing platform. It is shown here that the automated cell culturing platform is able to properly execute DMSO-free cryopreservation, thawing, plating, and cell maintenance. This demonstrated ability to perform these functions completely automated without a technician is a technical advancement in pluripotent stem cell culturing and may provide financial benefits within a cell culture laboratory.
Keywords
Description
University of Minnesota M.S. thesis. December 2019. Major: Stem Cell Biology. Advisors: James Dutton, Susan Keirstead. 1 computer file (PDF); v, 45 pages + 1 supplementary file.
Related to
Replaces
License
Series/Report Number
Funding information
Isbn identifier
Doi identifier
Previously Published Citation
Suggested citation
Johnson, Blake. (2019). Automation of Stem Cell Protocols. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/211697.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.