The Effect of Saturated Fatty Acids on Lipogenic Gene Expression in Rat Primary Hepatocytes
2009-04-08
Loading...
View/Download File
Persistent link to this item
Statistics
View StatisticsJournal Title
Journal ISSN
Volume Title
Title
The Effect of Saturated Fatty Acids on Lipogenic Gene Expression in Rat Primary Hepatocytes
Authors
Published Date
2009-04-08
Publisher
Type
Presentation
Abstract
Consumption of saturated fat has been associated with the development of obesity, diabetes, hepatic steatosis and related diseases. Additionally, animal studies have shown that consumption of a diet high in saturated fat results in a rapid upregulation of hepatic lipogenic genes. Thus, to determine if saturated fatty acids have a direct effect on hepatic gene expression, rat primary hepatocytes were cultured and treated with various fatty acids. RNA was harvested from the hepatocytes and cDNA was subsequently prepared to analyze for lipogenic gene expression. Lipogenic enzymes included fatty acid synthase (FAS), acetyl-coA carboxylase (ACC) isoforms alpha and beta, stearoyl-coenzyme A desaturase 1 (SCD1) and fatty acid elongase 6 (Elovl6). Although lipogenic gene expression was altered in response to the presence of insulin and glucose, saturated fatty acids did not appear to significantly upregulate gene expression of lipogenic genes. These data suggest that the effects of saturated fatty acids on lipogenic gene expression in the liver are not direct. Further experiments will explore whether signals from the adipose tissue or inflammatory signals from microphages are necessary for saturated fatty acids to cause changes in de novo lipogenesis.
Description
Additional contributor: Douglas Mashek (faculty mentor).
Related to
Replaces
License
Series/Report Number
Funding information
Isbn identifier
Doi identifier
Previously Published Citation
Suggested citation
Best, Amanda. (2009). The Effect of Saturated Fatty Acids on Lipogenic Gene Expression in Rat Primary Hepatocytes. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/50264.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.