Cultivation of Natural Killer Cell for Immunotherapy
2018-04
Loading...
View/Download File
Persistent link to this item
Statistics
View StatisticsJournal Title
Journal ISSN
Volume Title
Title
Cultivation of Natural Killer Cell for Immunotherapy
Authors
Published Date
2018-04
Publisher
Type
Thesis or Dissertation
Abstract
Natural Killer (NK) cells are a type of blood immune cells that are capable of lyse the infected or transformed cells without prior sensitization. Due to a small fraction of NK cells within human bodies, and the fact that the cells are not able to expand to lyse the target cells, cells were isolated from human blood, and were expanded. NK cells expanded without feeder cells showed only a small number of fold expansion. However, when the NK cells were co-cultivated with artificial Antigen Presenting Cells (aAPCs), NK cells showed a much greater number of fold expansion. Especially, NK cells co-cultured with K562 cells with membrane bound IL-21 (K562.mbIL21) showed about 10,000-fold expansion for 14 days cultivation. Continuous cultivation of NK cells with the K562.mbIL21 showed more than 100 billion-fold expansion for 30 days before it shows senescence. By employing a multivariate analysis technique, phenotypic changes during the activation and expansion of NK cells were captured. In addition, various kinetic parameters during the cultivation were identified to provide preliminary data for future research. It was found that NK cells could expand with dead aAPCs and their debris, showing a possibility of expanding NK cells without feeder cells since the K562 cells are cancerous, thus, they need to be completely removed for clinical use in promoting NK cells in the field of adoptive immunotherapy.
Keywords
Description
University of Minnesota M.S. thesis. April 2018. Major: Microbial Engineering. Advisor: Wei-Shou Hu. 1 computer file (PDF); vii, 91 pages.
Related to
Replaces
License
Series/Report Number
Funding information
Isbn identifier
Doi identifier
Previously Published Citation
Suggested citation
Kim, Hansol. (2018). Cultivation of Natural Killer Cell for Immunotherapy. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/198358.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.