Assessing Function Efficiency of AtWUS Through Genetic Modifications

Loading...

View/Download File

FINAL POSTER.pdf (1.22 MB)

Persistent link to this item

https://hdl.handle.net/11299/214000

Statistics

View Statistics

Journal Title

Journal ISSN

Volume Title

Title

Assessing Function Efficiency of AtWUS Through Genetic Modifications

Authors

Badey, Nikil, B

Published Date

2020-06

Publisher

Type

Presentation

Abstract

This research aims to optimize developmental transporter, AtWUS by removing the dimerization domain found in exon 2 of the protein. By removing the dimerization domain, we were able to achieve a more sustainable and efficient system of growth unlike previous model comparisons of AtWUS.

Keywords

Gene Editing

Cloning

Synthetic Biology

Genetic Engineering

Plant Biology

Genetics

Plant Engineering

Description

The genetic architecture of both well-characterized (WUS box, EAR-like, etc.) and less studied domains in WUS proteins are subject for genetic alteration. WUS has been shown as a bifunctional transcription factor that primarily acts as a repressor. By mutating the functional domains (WOX homeobox domain, WUS-box domain, EAR-like domain, acidic domain) of the WUS protein, their role can be determined in the regulation of stem cell identity. The WOX homeobox domain is a helix-loop-helix which is the DNA binding domain that allows for WUS to properly bind its DNA targets. WUS box and EAR-like are domains that allow WUS to function primarily as a repressor. Finally, the acidic region has been shown to be an activation domain that primarily acts during floral patterning. However, if mutated, it can eliminate the activities of WUS including the induction of expression of its downstream targets. Based on the activities of these functional domains WUS can thus be characterized as a repressor or activator. Overexpression of WUS causes uncontrolled cell division that is coordinated through its repressor domains. Ikeda et al. shows the WUS box as an essential feature for cell division functions. Through the usage of Green Fluorescence Protein tagging, as show through the work of Rodriguez et al., different WUS mutants were tagged and found that certain domains are required to localize WUS in the central zone of the meristem, creating a good mutation target. Through the removal of the dimerization domain or exon 2, the delocalization of WUS produces a variant that can induce more growths and shoots. To test the hypotheses, Fast-TrACC (Fast-Treated Agrobacterium Co-Culture) will be utilized using A. tumefaciens culture to deliver a luciferase reporter along with the developmental regulator WUS to promote meristem formation.

Related to

Replaces

License

Collections

UMTC Undergraduate Research Presentations and Papers (UROP)

Series/Report Number

Funding information

This research was supported by the Undergraduate Research Opportunities Program (UROP). Center for Precision Plant Genomics, University of Minnesota - Twin Cities.

Isbn identifier

Doi identifier

Previously Published Citation

Suggested citation

Badey, Nikil, B. (2020). Assessing Function Efficiency of AtWUS Through Genetic Modifications. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/214000.

Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.