Triggered Degradation of Polyacrylamide Gels for DNA Recovery

Loading...
Thumbnail Image

Persistent link to this item

Statistics
View Statistics

Journal Title

Journal ISSN

Volume Title

Title

Triggered Degradation of Polyacrylamide Gels for DNA Recovery

Published Date

2011-04-13

Publisher

Type

Presentation

Abstract

Polyacrylamide gels are commonly used in the analysis and separation of DNA by gel electrophoresis. In some protocols, once a sample of DNA undergoes gel electrophoresis, the DNA sample is recovered from the gel. A common method of doing this is to soak pieces of gel in buffer and wait for the DNA to diffuse out of the gel. This can take many hours or even days for DNA strands longer than 500 base pairs. It would be extremely useful to incorporate a chemically triggerable release mechanism into the polyacrylamide gel that would allow a researcher to decompose the gel network at will and recover the embedded DNA more quickly. We have developed a bis(acrylamide) crosslinker that contains a chemically cleavable group. Polyacrylamide gels made with this crosslinker (“EG2”) decompose quickly when exposed to a DNAcompatible reductants. In principle, due to the shortened time span, less stable nucleic acids such as RNA might be recovered with a reduced amount of decomposition of the actual RNA sample.

Description

Additional contributors:T. Andrew Taton; Jun Sung Kang (faculty mentor)

Related to

Replaces

License

Series/Report Number

Funding information

Isbn identifier

Doi identifier

Previously Published Citation

Suggested citation

Wagner, Kristen. (2011). Triggered Degradation of Polyacrylamide Gels for DNA Recovery. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/104437.

Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.