Optimization of Camelina Protein Extraction and Screening of Diverse Lines for Differences in Protein Profile, Structure, and Functionality

Abstract

Current consumer trends such as vegan and flexitarian diets and interest in sustainably sourced ingredients are driving the demand for plant proteins. Producers are seeking alternative plant proteins that are non-allergenic and non-GMO. Camelina sativa, a novel oilseed crop with several environmental benefits, is high in protein content (20-30%). The objectives of this research were: 1) Optimize protein extraction methodology to produce camelina protein isolates (CPI) with optimal yield, purity, structural characteristics, and functional properties; 2) Screen camelina lines for protein profile, structure, functionality, and nutritional quality to develop optimal lines for food use. This study showed that CPI with optimal purity, yield, color, and functionality can be produced using industry feasible extraction methods, thus advancing its commercialization as an alternative plant protein. The structural and functional characterization of the different camelina lines provided useful information to the camelina breeding programs that aim at developing camelina protein for food use.